LOCUS       sequence-183672-         909 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of E. coli LF82_364 CDS fused to mEmerald.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 909)
  AUTHORS   Collins A, Huett A
  TITLE     A multi-phenotypic imaging screen to identify bacterial effectors by
            exogenous expression in a HeLa cell line.
  JOURNAL   Sci Data. 2018 May 15;5:180081. doi: 10.1038/sdata.2018.81.
  PUBMED    29762554
REFERENCE   2  (bases 1 to 909)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Data.";
            date: "2018-05-15"; volume: "5:180081. doi"; pages: " 
            10.1038/sdata.2018.81"
COMMENT     Sequence Label: pCMV_mEm_4GS_LF82_364
FEATURES             Location/Qualifiers
     source          1..909
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    336..470
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(360..379)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     414..433
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(581..597)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(581..597)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(594..616)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    605..621
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(629..635,636..653,654..659))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     primer_bind     complement(815..832)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ggttccggtg gcggaggttc ccgaattcgt cgacggagat ctgatatgtt tgagcgtagc
       61 gttggtaacg ctaccaggcg cgtgaatgtt accccatttt atgtatggac atgtagtgaa
      121 aactacaact ttgtcgttcg ctttggcatc atcttcatta taaagacgga attttgcatc
      181 agcttctcat caggaaaaac aactaaaaaa tcaatcacaa tacttgtgca tttctttaaa
      241 cagtttttta aaactgactc cgggtatgga gctatgggta ttttctgtcc ccaatgcttt
      301 tgaatcggta ccctcgagaa gcttgcggcc gcggggatcc agacatgata agatacattg
      361 atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt
      421 gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca
      481 attgcattca ttttatgttt caggttcagg gggaggtgtg ggaggttttt tcggatcctc
      541 tagagtcgat ctgcaggcat gctagcttgg cgtaatcatg gtcatagctg tttcctgtgt
      601 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
      661 cctggggtgc ctaatgagtg agctaaactc acattaattg cgttgcgctc actgccccgc
      721 tttccagtcg gagaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
      781 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
      841 tcgttcggct gcggcgagcg gtatcagctc actcaaaggg cggtaatacg gttatccaca
      901 gaatcacgg
//
