LOCUS       sequence-183675-         901 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of E. coli LF82_774 CDS fused to mEmerald.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 901)
  AUTHORS   Collins A, Huett A
  TITLE     A multi-phenotypic imaging screen to identify bacterial effectors by
            exogenous expression in a HeLa cell line.
  JOURNAL   Sci Data. 2018 May 15;5:180081. doi: 10.1038/sdata.2018.81.
  PUBMED    29762554
REFERENCE   2  (bases 1 to 901)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Data.";
            date: "2018-05-15"; volume: "5:180081. doi"; pages: " 
            10.1038/sdata.2018.81"
COMMENT     Sequence Label: pCMV_mEm_4GS_LF82_774
FEATURES             Location/Qualifiers
     source          1..901
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    324..458
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(348..367)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     402..421
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(569..585)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(569..585)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(582..604)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    593..609
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(617..623,624..641,642..647))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    662..683
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(800..817)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ctcggcatgg acgagctgta caagcgaatt ggtggagggg gcggttccgg tggcggaggt
       61 tcccgaattc gtcgacggag atctgatatg cagtctgact ggcatcctgc ctacattatc
      121 gctgggctga aaaaacgtgg cacttcgctg tcagcgcttt cgcgtcaggc tgggctggcg
      181 tcatccacgc tggctaatgc gcttacccgc cgctggccca aaggcgaaag attgattgca
      241 gaagcgttag atgttgcgcc tgagaacatc tggccttcac gctatcgctg aatcggtacc
      301 ctcgagaagc ttgcggccgc ggggatccag acatgataag atacattgat gagtttggac
      361 aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg
      421 ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt
      481 ttatgtttca ggttcagggg gaggtgtggg aggttttttc ggatcctcta gagtcgatct
      541 gcaggcatgc tagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
      601 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
      661 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
      721 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
      781 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
      841 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatcn ggggataacg
      901 c
//
