LOCUS       sequence-183677-        1047 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of E. coli LF82_788 CDS fused to mEmerald.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1047)
  AUTHORS   Collins A, Huett A
  TITLE     A multi-phenotypic imaging screen to identify bacterial effectors by
            exogenous expression in a HeLa cell line.
  JOURNAL   Sci Data. 2018 May 15;5:180081. doi: 10.1038/sdata.2018.81.
  PUBMED    29762554
REFERENCE   2  (bases 1 to 1047)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Data.";
            date: "2018-05-15"; volume: "5:180081. doi"; pages: " 
            10.1038/sdata.2018.81"
COMMENT     Sequence Label: pCMV_mEm_4GS_LF82_788
FEATURES             Location/Qualifiers
     source          1..1047
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    815..949
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(839..858)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     893..912
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 gcatggncga gctgtacaag cgaattggtg gagggggcgg ttccggtggc ggaggttccc
       61 gaattcgtcg acggagatct gatatggtta tgttagtgga tgactgtcca cgatgtggat
      121 cacaaaaaat agcgtttgat gttaatgggc tgaattgcac tagggtttac aatgcattgg
      181 gcgggggtaa aacatacgag tacgaggtct attgtgtttg ccgagagtgt cataaaacga
      241 caatgtttct ttgcagacct ttaacgaaaa ataaaacttt agatggctat aattgggaaa
      301 gtggaatatt cggcttaaag gaagttgcag aggttgttag gcctatatca ccggcagact
      361 tagcagtaga ggagccacca gagttcttac ctgagcatat caatagtgca tatgaggaag
      421 gggcgaaatg tttggctata ggttgttata atgcagcagc aaccatgttt aggctttgcc
      481 ttgattatgc cacaaaagga cttcttcctg atggggagca agggcctgcg caaaaaataa
      541 ggagaagcct agggttaaga atggagtggt tatttgataa tcatctactg cctgaagctt
      601 taagggaact agctgaatgc gttaaagatg acggaaatga tggagcgcat gaaggtattt
      661 tggataaagc tgctgctgag gatcttgaag attttaccta tcttttttta gaacggcttt
      721 acactgaacc tcaacgcctt atcgaagcca agacaaggcg tgaacaaaga agaaacaaat
      781 gaatcggtac cctcgagaag cttgcggccg cggggatcca gacatgataa gatacattga
      841 tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg
      901 tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta acaacaacaa
      961 ttgcattcat tttatgtttc aggttcnngg gaggtgtggg aggttttttt cgnatcctct
     1021 agagtcgatn ntgcaggcat gctagct
//
