LOCUS       sequence-183679-         897 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of E. coli LF82_789 CDS fused to mEmerald.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 897)
  AUTHORS   Collins A, Huett A
  TITLE     A multi-phenotypic imaging screen to identify bacterial effectors by
            exogenous expression in a HeLa cell line.
  JOURNAL   Sci Data. 2018 May 15;5:180081. doi: 10.1038/sdata.2018.81.
  PUBMED    29762554
REFERENCE   2  (bases 1 to 897)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Data.";
            date: "2018-05-15"; volume: "5:180081. doi"; pages: " 
            10.1038/sdata.2018.81"
COMMENT     Sequence Label: pCMV_mEm_4GS_LF82_789
FEATURES             Location/Qualifiers
     source          1..897
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    732..866
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(756..775)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     810..829
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 ctcggcatgg acgagctgta cnagcgaatt ggtggagggg gcggttccgg tggcggaggt
       61 tcccgaattc gtcgacggag atctgatatg gatttagtct ttaaaattct ggcttcgttg
      121 ggtggggtgt cttttgttgc atctggcata tttgtttgga tcgggaaagt ttatttagaa
      181 agatataagt cgcggctaaa caaagatatt gctgaatttc aatcacaact gagtgcaact
      241 aatgaaagaa taaaggctaa gttagacaat tctgtttatg taacgaaagc gtattttgat
      301 aaggagttat cagcatatag tctcatttgg aattcgatgt ttgaaaccag agaaagcgtg
      361 cttaagctga ggcctgcgct ggatcatttt gaccccaacg aaccatttga agagagaaaa
      421 tttagaaggt tgaaagtttt tttcgatgca tttaatactt ttgttacaag tgttgagtct
      481 aacaaaccgt tcatctcacc agaggtttat ataatcttgg accgtttccg gaaggaatgc
      541 ctttcagagt caatatcgtt tcagcatggc gatccagaat ttgactggca aaattattgg
      601 aaagaggcag agttgaaccg tacaaccatc accaagcttt ttgatgagac gtgtgatgca
      661 attcgagaca ggatgcacac attaactgtg gttacgtgaa tcggtaccct cgagaagctt
      721 gcggccgcgg ggatccagac atgataagat acattgatga gtttggacaa accacaacta
      781 gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa
      841 ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttc
//
