LOCUS       sequence-183681-        1033 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of E. coli LF82_803 CDS fused to mEmerald.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1033)
  AUTHORS   Collins A, Huett A
  TITLE     A multi-phenotypic imaging screen to identify bacterial effectors by
            exogenous expression in a HeLa cell line.
  JOURNAL   Sci Data. 2018 May 15;5:180081. doi: 10.1038/sdata.2018.81.
  PUBMED    29762554
REFERENCE   2  (bases 1 to 1033)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Data.";
            date: "2018-05-15"; volume: "5:180081. doi"; pages: " 
            10.1038/sdata.2018.81"
COMMENT     Sequence Label: pCMV_mEm_4GS_LF82_803
FEATURES             Location/Qualifiers
     source          1..1033
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    357..491
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(381..400)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     435..454
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(602..618)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(602..618)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(615..637)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    626..642
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(650..656,657..674,675..680))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    695..716
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(834..851)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ctcngcatgg acgagctgta cnagcgaatt ggtggagggg gcggttccgg tggcggaggt
       61 tcccgaattc gtcgacggag atctgatatg aacgatgccc agaccagcgc atttaaggtg
      121 gcaagtggca acgccgatcc cgcactgctg agcaaagtgt tcattggtgc gctgattgct
      181 ctgctgattt tgtgggtggg atggggtttt ctgcatgttt atcgggggta cgcggcaggt
      241 cacatcaaag agcaggcact ggtgcgtttt gccatccgtt cagtccttct catcattatc
      301 gctatctatc tcttcgccag ctgaatcggt accctcgaga agcttgcggc cgcggggatc
      361 cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa
      421 aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca
      481 ataaacaagt taacaacaac aattgcattc attttatgtt tcaggttcag ggggaggtgt
      541 gggaggtttt ttcggatcct ctagagtcga tctgcaggca tgctagcttg gcgtaatcat
      601 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
      661 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
      721 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
      781 tcggccaacg cgcggggaga ggcggtttgc gtattggggc gctcttccgc ttcctcgctc
      841 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
      901 gtaatacgnt tatccacaga atcngggatn acgcaggaaa ganatgtgag caaaaggcca
      961 gcaaangcca ggaaccgtaa aaggccgcgt tgctggcgtt tttccatagg ntccgccccc
     1021 cngacgagca tca
//
