LOCUS       sequence-183683-         917 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of E. coli LF82_619 CDS fused to mEmerald.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 917)
  AUTHORS   Collins A, Huett A
  TITLE     A multi-phenotypic imaging screen to identify bacterial effectors by
            exogenous expression in a HeLa cell line.
  JOURNAL   Sci Data. 2018 May 15;5:180081. doi: 10.1038/sdata.2018.81.
  PUBMED    29762554
REFERENCE   2  (bases 1 to 917)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Data.";
            date: "2018-05-15"; volume: "5:180081. doi"; pages: " 
            10.1038/sdata.2018.81"
COMMENT     Sequence Label: pCMV_mEm_4GS_LF82_619
FEATURES             Location/Qualifiers
     source          1..917
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    364..498
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(388..407)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     442..461
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(609..625)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(609..625)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(622..644)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    633..649
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(657..663,664..681,682..687))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    702..723
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(840..857)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gaggggcggt tccggtggcg gaggttcccg aattcgtcga cggagatctg atatgtccac
       61 gtttaatacg cgtaaagata gggatcgtaa tggtaaagcg aagccgcata agcattcagg
      121 gatcccaaca cttacaggta aatggcgtca cccttcaatg gattgttggg taaactacag
      181 tacaccgggc gatcatgaat ggaagcggct ctatactacg cttcgccgtc gcgcagatga
      241 tcgacgccaa tgtcatcggg taatgcaggg ggatgatccg gaaggaatcg tctggttccc
      301 ggactgttat ccacaaattt actactactg aatcggtacc ctcgagaagc ttgcggccgc
      361 ggggatccag acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag
      421 tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata
      481 agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg
      541 gaggtgtggg aggttttttc ggatcctcta gagtcgatct gcaggcatgc tagcttggcg
      601 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
      661 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
      721 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
      781 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
      841 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
      901 aaggcggtaa tacggta
//
