LOCUS       sequence-183684-         970 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of E. coli LF82_312 CDS fused to mEmerald.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 970)
  AUTHORS   Collins A, Huett A
  TITLE     A multi-phenotypic imaging screen to identify bacterial effectors by
            exogenous expression in a HeLa cell line.
  JOURNAL   Sci Data. 2018 May 15;5:180081. doi: 10.1038/sdata.2018.81.
  PUBMED    29762554
REFERENCE   2  (bases 1 to 970)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Data.";
            date: "2018-05-15"; volume: "5:180081. doi"; pages: " 
            10.1038/sdata.2018.81"
COMMENT     Sequence Label: pCMV_mEm_4GS_LF82_312
FEATURES             Location/Qualifiers
     source          1..970
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    453..587
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(477..496)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     531..550
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(698..714)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(698..714)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(711..733)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    722..738
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(746..752,753..770,771..776))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    791..812
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(930..947)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ctcggcntgg nngagctgta caagcgaatt ggtggagggg gcggttccgg tggcggaggt
       61 tcccgaattc gtcgacggag atctgatatg gacagagggg aaatctggct ggtttcactg
      121 gatccgattg caggtcacga gcaaagcgga aaacgtccgg tgcttatcgt atcgaaggca
      181 tcgtttaaca agctgacccg gctacccgtt gttgttcccg ttaccagcgg cggaaacttc
      241 gcccgtgcag ccggttttac cgtctcactg gacggtgcgg gaacaaaaac aacgggcgtt
      301 attcgctgcg accagcccag aaccattgat atgggagccc ggagcggtaa gcgactggaa
      361 cgtatacccg atgctgtcgt gaatgaagtg ctggcccgac tggaggccat tctgagctga
      421 atcggtaccc tcgagaagct tgcggccgcg gggatccaga catgataaga tacattgatg
      481 agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg
      541 atgctattgc tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt
      601 gcattcattt tatgtttcag gttcaggggg aggtgtggga ggttttttcg gatcctctag
      661 agtcgatctg caggcatgct agcttggcgt aatcatggtc atagctgttt cctgtgtgaa
      721 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
      781 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
      841 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcn
      901 gntttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
      961 cggctgcggc
//
