LOCUS       sequence-183687-         966 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of E. coli LF82_785 CDS fused to mEmerald.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 966)
  AUTHORS   Collins A, Huett A
  TITLE     A multi-phenotypic imaging screen to identify bacterial effectors by
            exogenous expression in a HeLa cell line.
  JOURNAL   Sci Data. 2018 May 15;5:180081. doi: 10.1038/sdata.2018.81.
  PUBMED    29762554
REFERENCE   2  (bases 1 to 966)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Data.";
            date: "2018-05-15"; volume: "5:180081. doi"; pages: " 
            10.1038/sdata.2018.81"
COMMENT     Sequence Label: pCMV_mEm_4GS_LF82_785
FEATURES             Location/Qualifiers
     source          1..966
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    526..660
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(550..569)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     604..623
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(771..787)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(771..787)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(784..806)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    795..811
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(819..825,826..843,844..849))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    864..885
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 cggcatggac gagctgtaca agcgaattgg tggagggggc ggttccggtg gcggaggttc
       61 ccgaattcgt cgacggagat ctgatatgat gccagttagc cgttatctct gcattttcat
      121 aaacgttggt ttgggagaag cggcaaaacg aaatgtgggt aacgagcaaa accaaattcc
      181 ggatatggcg gcgtttgcca gttcactttc atcaacgggt tttcaaaaac tcccttcagg
      241 tctgattatt cagtggggta ttgtcagtgg agcatcaaac tatacggtga cttacccggt
      301 aacattccca aatcgttcac ttgcgctgtt ggctgtgcca catacaacgt cggtggctgg
      361 tatatctgca atgggcatag cgaactgttc tgatatcagc aaatcacagt tctatataat
      421 tgttggcggt atatctcagg gagaaattgt caaatatgaa aggtcctgtt tttgggtagc
      481 aatcggtgta tgaatcggta ccctcgagaa gcttgcggcc gcggggatcc agacatgata
      541 agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt
      601 tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt
      661 aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg ggangttttt
      721 tcggatcctc tagagtcgat ctgcangcat gctagcttgg cgtaatcatg gtcatagctg
      781 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
      841 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
      901 ctgcccgctt tccagtnggg aaanctgtcg tgccagctgc attaatgaat cgnncaacgc
      961 gcgggg
//
