LOCUS       sequence-183688-         982 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of E. coli LF82_682 CDS fused to mEmerald.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 982)
  AUTHORS   Collins A, Huett A
  TITLE     A multi-phenotypic imaging screen to identify bacterial effectors by
            exogenous expression in a HeLa cell line.
  JOURNAL   Sci Data. 2018 May 15;5:180081. doi: 10.1038/sdata.2018.81.
  PUBMED    29762554
REFERENCE   2  (bases 1 to 982)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Data.";
            date: "2018-05-15"; volume: "5:180081. doi"; pages: " 
            10.1038/sdata.2018.81"
COMMENT     Sequence Label: pCMV_mEm_4GS_LF82_682
FEATURES             Location/Qualifiers
     source          1..982
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    460..594
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(484..503)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     538..557
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(705..721)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(705..721)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(718..740)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    729..745
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    800..821
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(940..957)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tggagggggc ggttccggtg gcggaggttc ccgaattcgt cgacggagat ctgatatgtt
       61 ttgttctgca ttatcttctc tgcgtttttc ttttcgtctc tggtgtgcgc gggttctggc
      121 cgttgccctg accggaattt cggctgcccg ggcgggtttg cccaccgttg aaggcccgaa
      181 aagcagcacc gacagctctt tctacggtca gatctcggga tacctcaatg acggtgttgt
      241 gctgggcgga ttaattcttg cggcagttgc catgctggcc gtcggtaacg ccattattgc
      301 cacctttgcc gaagttcgtg acgggaaatc aacctgggct aaattcggga tgctttgcgt
      361 tgtgggggtg atcctgatcg tcgtagttat ctggctggcg tctaaggccg caacggttct
      421 gctctgaatc ggtaccctcg agaagcttgc ggccgcgggg atccagacat gataagatac
      481 attgatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa
      541 atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac
      601 aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt tttttcggat
      661 cctctagagt cgatctgcag gcatgctagc ttggcgtaat catggtcata gctgtttcct
      721 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaaa gcataaaagt
      781 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
      841 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggg ccaacgcgcg
      901 gggagaggcg ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
      961 ctcgggtcgt tcggctgcgg cg
//
