LOCUS       sequence-183689-         945 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of E. coli LF82_683 CDS fused to mEmerald.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 945)
  AUTHORS   Collins A, Huett A
  TITLE     A multi-phenotypic imaging screen to identify bacterial effectors by
            exogenous expression in a HeLa cell line.
  JOURNAL   Sci Data. 2018 May 15;5:180081. doi: 10.1038/sdata.2018.81.
  PUBMED    29762554
REFERENCE   2  (bases 1 to 945)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Data.";
            date: "2018-05-15"; volume: "5:180081. doi"; pages: " 
            10.1038/sdata.2018.81"
COMMENT     Sequence Label: pCMV_mEm_4GS_LF82_683
FEATURES             Location/Qualifiers
     source          1..945
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    468..602
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(492..511)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     546..565
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(713..729)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(713..729)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(726..748)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    737..753
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(763..768,769..786,787..792))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    807..828
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 ggagggggcg gttccggtgg cggaggttcc cgaattcgtc gacggagatc tgatatggcg
       61 actatcgaat ttatgcctga caggctgaat gctgagccgg tggtgtttcg tggattcacc
      121 acgccggaac tgggatttac cgcgctgttc agcgtactgg gcggcctcgt ggtcgccata
      181 ccagttctgc catttctcgg ctggatagcc tttccgaccg tggcgctgct gatgccgctg
      241 gtcacggtta tttttggcgg caggttaatg gcgcgtttta agcgcggtaa gccagaaaat
      301 tacctctatc gcaggctgga tctgcacatc agccagtttg gtttgggtac aaagggactg
      361 gtgcaaagca gtcgggtgct ggcgctacgt cgtcaccggc tggttgtccg ccgccaggag
      421 gaaatccatg agtgaatcgg taccctcgag aagcttgcgg ccgcggggat ccagacatga
      481 taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta
      541 tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag
      601 ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggaggtg tgggaggttt
      661 tttcggatcc tctagagtcg atctgcaggc atgctagctt ggcgtaatca tggtcatagc
      721 tgtttcctgt gtgaaattgt tatccgctca caattccaca caaacatacg agccggaagc
      781 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
      841 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcgggcca
      901 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttc
//
