LOCUS       sequence-183693-        1052 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of E. coli LF82_147 CDS fused to mEmerald.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1052)
  AUTHORS   Collins A, Huett A
  TITLE     A multi-phenotypic imaging screen to identify bacterial effectors by
            exogenous expression in a HeLa cell line.
  JOURNAL   Sci Data. 2018 May 15;5:180081. doi: 10.1038/sdata.2018.81.
  PUBMED    29762554
REFERENCE   2  (bases 1 to 1052)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Data.";
            date: "2018-05-15"; volume: "5:180081. doi"; pages: " 
            10.1038/sdata.2018.81"
COMMENT     Sequence Label: pCMV_mEm_4GS_LF82_147
FEATURES             Location/Qualifiers
     source          1..1052
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    309..443
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(333..352)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     387..406
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(554..570)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(554..570)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(567..589)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    578..594
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(602..608,609..626,627..632))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    647..668
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(785..802)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ctcggcatgg acgagctgta cnagcgaatt ggtggagggg gcggttccgg tggcggaggt
       61 tcccgaattc gtcgacggag atctgatatg gcgatgacgc atcctcacga taatatccgg
      121 gtaggcgcga tcactttcgt ctactccgtt acaaagcgag gctgggtatt tcccggcctt
      181 tctgttatcc aaaatccact gaaagcccag cggctggctg aggagataaa taataaacgg
      241 ggggctgtat gcacaaagca tctcccgttg agttgaatcg gtaccctcga gaagcttgcg
      301 gccgcgggga tccagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa
      361 tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca
      421 ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc
      481 agggggaggt gtgggaggtt ttttcggatc ctctagagtc gatctgcagg catgctagct
      541 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
      601 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
      661 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
      721 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
      781 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
      841 actcaaaggc ggtaatacgg ttatccacag aatcngggga taacgcnnna agaacatgtg
      901 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
      961 taggctccgc cccccngacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcnaaa
     1021 ccnacaggac tanaagatac caggcnttnc cc
//
