LOCUS       sequence-183694-         959 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of E. coli LF82_696 CDS fused to mEmerald.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 959)
  AUTHORS   Collins A, Huett A
  TITLE     A multi-phenotypic imaging screen to identify bacterial effectors by
            exogenous expression in a HeLa cell line.
  JOURNAL   Sci Data. 2018 May 15;5:180081. doi: 10.1038/sdata.2018.81.
  PUBMED    29762554
REFERENCE   2  (bases 1 to 959)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Data.";
            date: "2018-05-15"; volume: "5:180081. doi"; pages: " 
            10.1038/sdata.2018.81"
COMMENT     Sequence Label: pCMV_mEm_4GS_LF82_696
FEATURES             Location/Qualifiers
     source          1..959
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    533..667
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(557..576)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     611..630
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(778..794)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(778..794)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(791..813)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    802..818
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(826..832,833..850,851..856))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    871..892
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 gagggggcgg ttccggtggc ggaggttccc gaattcgtcg acggagatct gatatggaag
       61 gcgtaataaa atggttcagt gaagagaaag ggtacggatt catcactgcc gcgaataata
      121 tagattacta ttttcatgtc agtgatataa aaggctcatt gcttcctgaa aaggggcaaa
      181 atgctcattt taatgcagaa tcgacatcta agggccatag ggcaaaaaac attactctta
      241 caaagaataa tattgcatca gataatcgtc tggatttttg caaaaactgt aataaaaaaa
      301 ttattccaag aatgattacc tacaggggtg aaccacaaaa aaccgtttgc ccttactgtg
      361 caacagtaat aaagaatttc gatgataatt ttttgggtga aatcattgca gcgatatttt
      421 attgtttcac ttccatgata aaaatactct ttgaatttat cacatggata tttagcatca
      481 tagctaacaa aaggaaatga atcggtaccc tcgagaagct tgcggccgcg gggatccaga
      541 catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg
      601 ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa
      661 acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga
      721 ggttttttcg gatcctctag agtcgatctg caggcatgct agcttggcgt aatcatggtc
      781 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
      841 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
      901 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcg
//
