LOCUS       sequence-183699-        1008 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of E. coli LF82_233 CDS fused to mEmerald.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1008)
  AUTHORS   Collins A, Huett A
  TITLE     A multi-phenotypic imaging screen to identify bacterial effectors by
            exogenous expression in a HeLa cell line.
  JOURNAL   Sci Data. 2018 May 15;5:180081. doi: 10.1038/sdata.2018.81.
  PUBMED    29762554
REFERENCE   2  (bases 1 to 1008)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Data.";
            date: "2018-05-15"; volume: "5:180081. doi"; pages: " 
            10.1038/sdata.2018.81"
COMMENT     Sequence Label: pCMV_mEm_4GS_LF82_233
FEATURES             Location/Qualifiers
     source          1..1008
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    492..626
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(516..535)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     570..589
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(737..753)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(737..753)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(750..772)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    761..777
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(785..791,792..809,810..815))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    830..851
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(968..985)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ctcngnatgg acgagctgta caagcgaatt ggtggagggg gcggttccgg tggcggaggt
       61 tcccgaattc gtcgacggag atctgatatg agtgaaaagc gtcatggcag agaagaaatt
      121 cgtcttcaca ttgtctgcga tatccctgat gaacttattg atttcacgtt tgaatggaaa
      181 gggctgaaga aattatgcat ggcagtctcc tttcgttcaa taataacaga acaaaagaaa
      241 gagccagaaa tgacggtcag atattatatc agttctgctg atttaaccgc agagaagttc
      301 gccacagcaa tccgaaacca ctggtacgtg gagaataagc tgtactggcg tctggacgtg
      361 gtaatgaatg aagacgacag caaaataaga agaggaaact cagcagaatt atttttaggg
      421 atacggcata tcgctattca gggcagggtt aagacgtgaa tcggtaccct cgagaagctt
      481 gcggccgcgg ggatccagac atgataagat acattgatga gtttggacaa accacaacta
      541 gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa
      601 ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg
      661 ttcaggggga ggtgtgggag gttttttcgg atcctctaga gtcgatctgc aggcatgcta
      721 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
      781 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
      841 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
      901 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
      961 ccgcttcctc gctcactgac tcgctncgct cggncgttcg nctgcggc
//
