LOCUS       sequence-183700-        1048 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of E. coli LF82_251 CDS fused to mEmerald.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1048)
  AUTHORS   Collins A, Huett A
  TITLE     A multi-phenotypic imaging screen to identify bacterial effectors by
            exogenous expression in a HeLa cell line.
  JOURNAL   Sci Data. 2018 May 15;5:180081. doi: 10.1038/sdata.2018.81.
  PUBMED    29762554
REFERENCE   2  (bases 1 to 1048)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Data.";
            date: "2018-05-15"; volume: "5:180081. doi"; pages: " 
            10.1038/sdata.2018.81"
COMMENT     Sequence Label: pCMV_mEm_4GS_LF82_251
FEATURES             Location/Qualifiers
     source          1..1048
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    645..779
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(669..688)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     723..742
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(889..905)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(889..905)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(902..924)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     promoter        complement(join(937..943,944..961,962..967))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    982..1002
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 ctcggcatgg acgagctgta caagcgaatt ggtggagggg gcggttccgg tggcggaggt
       61 tcccgaattc gtcgacggag atctgatatg ctaccgggaa tggatggtta tcaggtactg
      121 gaaggcgtcc gggccagaaa acagaccccc gtgctgatgc tggcagcacg gggctcggtc
      181 gacgaacgcg tcaaagggct gcgtctggga gcagatgatt atctccctaa acccttttca
      241 ctcattgaat tggtggcgcg tattcaggcg ttggtgcgtc gccgcccttc ggatggcgca
      301 gacatcactc agcttcatat tcaggatctg catctcgact tactggcgcg cagggtattt
      361 cgcggtggaa cgagactgga actaaccgcg aaagagtttt ccctgttgag tttgctcgct
      421 cgccatcaag gggagatcct gtcaaagatg atgattgccg aacaagtctg ggacatgaat
      481 tttgatagtg atgccaatgt ggttgaagtc gctattaaac gtttacgcgc caaagttgat
      541 gccccattcg acgttaagtt gcttcatacc gttcgcggca tggggtatgt cctcgaagtc
      601 cggcctgagt gaatcggtac cctcgagaag cttgcggccg cggggatcca gacatgataa
      661 gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt
      721 gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta
      781 acaacaacaa ttgcattcat tttatgtttc angttcangg ggaggtgtgg gangtttttt
      841 cggatcctct agagtcgatc tgcnncatgc tagcttggcg taatcatggt catagctgtt
      901 tcctgtgtga aattgttatc cgctcanaat tccacacaac atacgagccg gaagcataaa
      961 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttnattgcgt tgcgctcact
     1021 gnccgntttn cagtcgggaa anctgncg
//
