LOCUS       sequence-183701-         756 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of E. coli LF82_769 CDS fused to mEmerald.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 756)
  AUTHORS   Collins A, Huett A
  TITLE     A multi-phenotypic imaging screen to identify bacterial effectors by
            exogenous expression in a HeLa cell line.
  JOURNAL   Sci Data. 2018 May 15;5:180081. doi: 10.1038/sdata.2018.81.
  PUBMED    29762554
REFERENCE   2  (bases 1 to 756)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Data.";
            date: "2018-05-15"; volume: "5:180081. doi"; pages: " 
            10.1038/sdata.2018.81"
COMMENT     Sequence Label: pCMV_mEm_4GS_LF82_769
FEATURES             Location/Qualifiers
     source          1..756
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    568..702
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(592..611)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     646..665
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 ctggaggggc ggttccggtg gcggaggttc ccgaattcgt cgacggagat ctgatatgat
       61 taagaatata caatcaaggc ttaccccgat atctcatgaa tatccaagtt gtgatgagtg
      121 ctatgctagc ctcttgattt acacaggtaa tactcaccct aatgaaataa gtattcactt
      181 agaacttgag ccaacatcaa tcaatataaa aggagatgaa attattactc tatcgggaaa
      241 gaaaagatat gcaaaaaatt catattggat gctctcgtca gaaaatctag tgaactcgaa
      301 agatattcga gaacatctgg attggctact tcacaaactt ggacctaaat catcaaatat
      361 tgcaaaattg caagcattag aaaatatttc aatgggtatt gactgtacct ggagatcatt
      421 gaattggcac ggtgggccaa ctttatggcc tgagcagatg aaaataatgg ctaatcttgg
      481 attggagtgt tcatttgata tttatttcgt cggcaatgaa gatggcaatc tttgaatcgg
      541 taccctcgag aagcttgcgg ccgcggggat ccagacatga taagatacat tgatgagttt
      601 ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct
      661 attgctttat ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt
      721 cattttatgt ttcaggttca gggggagtgt ggggag
//
