LOCUS       sequence-183705-         823 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of E. coli LF82_782 CDS fused to mEmerald.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 823)
  AUTHORS   Collins A, Huett A
  TITLE     A multi-phenotypic imaging screen to identify bacterial effectors by
            exogenous expression in a HeLa cell line.
  JOURNAL   Sci Data. 2018 May 15;5:180081. doi: 10.1038/sdata.2018.81.
  PUBMED    29762554
REFERENCE   2  (bases 1 to 823)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Data.";
            date: "2018-05-15"; volume: "5:180081. doi"; pages: " 
            10.1038/sdata.2018.81"
COMMENT     Sequence Label: pCMV_mEm_4GS_LF82_782
FEATURES             Location/Qualifiers
     source          1..823
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    278..412
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(302..321)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     356..375
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(523..539)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(523..539)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(536..558)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    547..563
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(571..577,578..595,596..601))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    616..637
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(754..771)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ccggtggcgg aggttcccga attcgtcgac ggagatctga tatgagtaag gagacttatc
       61 ggcaacgacg ccggtatgtt tctaatatgt tacatattat cggggacatt gttccagaca
      121 acaatctgtt ttattcaatg ggtatgatag acacaggctt tggaacgaaa tcatggcgaa
      181 ccgcgcggac ttcgggtaat ctgcgcgctt cgcgcagcgc tggtggagaa aagcatgaac
      241 gatgaatcgg taccctcgag aagcttgcgg ccgcggggat ccagacatga taagatacat
      301 tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat
      361 ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag ttaacaacaa
      421 caattgcatt cattttatgt ttcaggttca gggggaggtg tgggaggttt tttcggatcc
      481 tctagagtcg atctgcaggc atgctagctt ggcgtaatca tggtcatagc tgtttcctgt
      541 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
      601 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
      661 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
      721 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
      781 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gta
//
