LOCUS       sequence-183706-         915 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of E. coli LF82_783 CDS fused to mEmerald.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 915)
  AUTHORS   Collins A, Huett A
  TITLE     A multi-phenotypic imaging screen to identify bacterial effectors by
            exogenous expression in a HeLa cell line.
  JOURNAL   Sci Data. 2018 May 15;5:180081. doi: 10.1038/sdata.2018.81.
  PUBMED    29762554
REFERENCE   2  (bases 1 to 915)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Data.";
            date: "2018-05-15"; volume: "5:180081. doi"; pages: " 
            10.1038/sdata.2018.81"
COMMENT     Sequence Label: pCMV_mEm_4GS_LF82_783
FEATURES             Location/Qualifiers
     source          1..915
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    558..692
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(582..601)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     636..655
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(802..818)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(802..818)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(815..837)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    826..842
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(850..856,857..874,875..880))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
ORIGIN
        1 ttcggtggcg gaggttcccg aattcgtcga cggagatctg atatggaaga acttcacttt
       61 gtttacatca atgcaaatgg tcgtatcggt gttcactcaa tacagagcat cagttatagc
      121 gaaaatcaca tacagggcat ttgtaagaac accgatcgaa taaaaacctt ccgaaaagac
      181 cgcattctta aacaatacga ttcacaagaa caagccattc aggaatgcgc gtcattcctc
      241 cccgaaaact actcacatct cactaagcag tcaggtccga aaaaaaatac attcgatgtg
      301 tgtttcaccg gatttaagaa agcagataaa gaaaggttgg ttgataaggc gaatgaacaa
      361 ggattaacgg taagaacatc tgtaacccaa aaccttcaga tgctctgttg cggttacaat
      421 gcaggcccat caaaagtatc ggcagccagg atgaaaggca ctatcatcat agatgagcct
      481 ggctttatac attttcttga aacgggtgag atcccagatg aatgaatcgg taccctcgag
      541 aagcttgcgg ccgcggggat ccagacatga taagatacat tgatgagttt ggacaaacca
      601 caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat
      661 ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt
      721 ttcaggttca gggggaggtg tgggaggttt tttcggatcc tctagagtcg atctgcagca
      781 tgctagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
      841 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctgggggt gcctaatgag
      901 tgagctaaac tcaca
//
