LOCUS       sequence-183709-         975 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of E. coli LF82_582 CDS fused to mEmerald.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 975)
  AUTHORS   Collins A, Huett A
  TITLE     A multi-phenotypic imaging screen to identify bacterial effectors by
            exogenous expression in a HeLa cell line.
  JOURNAL   Sci Data. 2018 May 15;5:180081. doi: 10.1038/sdata.2018.81.
  PUBMED    29762554
REFERENCE   2  (bases 1 to 975)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Data.";
            date: "2018-05-15"; volume: "5:180081. doi"; pages: " 
            10.1038/sdata.2018.81"
COMMENT     Sequence Label: pCMV_mEm_4GS_LF82_582
FEATURES             Location/Qualifiers
     source          1..975
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    810..944
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(834..853)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     888..907
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 ctcggcatgg acgagctgta caagcgaatt ggtggagggg gcggttccgg tggcggaggt
       61 tcccgaattc gtcgacggag atctgatatg gcatttcccg ataactacaa ccgtctgttt
      121 ataccacggc aaaaaactaa agataataat ggcctcaaaa cactcaatag taaagcggtt
      181 ggcagactgc ttgctgctgg cggcatatac aatgggaata tcgaaggatt cagggaaaca
      241 gcaaacaagt tgggaggaga tgcacctgct ggttacgatc aagttatgga taacaaaggg
      301 ttactcatcg ctggtgcttc aatagttgct ggagttgttc tgggaagaat gagattccct
      361 gatttggaat catttgagca tttcggagcc agaggagcag tatcaggaag accatttaat
      421 cccgaccttg ccggtggtcc aatagaaaac ctgacgatag atggcgtaac aatcaatagg
      481 gaaggtatcg ctattgttga aaagcatatc tccagatttg atcatgatcc agctaatgat
      541 gtaatgattt caaggctaaa aaaaatagca aataaagaat tgcttcctga aaaatacgat
      601 ctgaactact atactcatga atgccgcgag tatcagcgtt attgtaattt aggatgggaa
      661 acaggtgaac cgaatggttt agatggatat gaattatgga ataatgcaca tactgcaact
      721 ctagaagact ttaaaattaa agatactgac ttatttcatc cggatgcaaa aaaatgaatc
      781 ggtaccctcg agaagcttgc ggccgcgggg atccagacat gataagatac attgatgagt
      841 ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg
      901 ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca
      961 ttcattttat gtttc
//
