LOCUS       sequence-183710-        1048 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of E. coli LF82_728 CDS fused to mEmerald.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1048)
  AUTHORS   Collins A, Huett A
  TITLE     A multi-phenotypic imaging screen to identify bacterial effectors by
            exogenous expression in a HeLa cell line.
  JOURNAL   Sci Data. 2018 May 15;5:180081. doi: 10.1038/sdata.2018.81.
  PUBMED    29762554
REFERENCE   2  (bases 1 to 1048)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Data.";
            date: "2018-05-15"; volume: "5:180081. doi"; pages: " 
            10.1038/sdata.2018.81"
COMMENT     Sequence Label: pCMV_mEm_4GS_LF82_728
FEATURES             Location/Qualifiers
     source          1..1048
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ctcggcatgg acgagctgta caagcgaatt ggtggagggg gcggttccgg tggcggaggt
       61 tcccgaattc gtcgacggag atctgatatg caatatttaa aaaaacgctt ttctttgatt
      121 gaactgaatg tgtttatccc agcaatatta tttattgcgg taatcattct gtgtttgacg
      181 atatatccac aagataccag ccggtatata aatgaaatac atcatttttt aacctgggaa
      241 atgggcggga tttttctggt catgactttt ctggttgtac tttgttgtct gtggctggcc
      301 ttctcacgtt atggcgatat cttgttaggt cagtcgggag aaaaacctga cttcagcttg
      361 ttaacctggc ttggacttat ttttacttct ggaacggggg gcagcttgtt atatctggcc
      421 tctgtagagt ggatttggat cattcagcaa ccgccttttg gcgcaacagc aggaagcgct
      481 caggctgctc gttgggcctc tgcttatggc atgttccatt gggggccgtc cgcatgggca
      541 tggtatttga tttgcgccgt tcctattggt tggtttatgc atgttaagaa aacaaactca
      601 ttaaaggtca gtgatttatg tcgtggatgt ttgggagcgc gtgcggatgg tttttgcggg
      661 cattgtgtga attttttcta catgtttggt ttgctcggcg gcgcggtaac gtctctggcg
      721 ctgggaacgc cgatgatttc tgccgtattt tgccatgtgt tccatctgga tcctgccggg
      781 cagtttatca atgtcatggt tatttttatc tggacgctag taccattatt cattctcttt
      841 tttggactta aaaaaggtgt ggcatgggcc agtaactgga atattcgtgc tgacattttg
      901 atgctactgg caatactgat ttgtggacca acggcattta tacttaatca atcaattgat
      961 ggtctcggnc tgatgctgca aaattttgta ncgatgantt tnagtaccga tgctantngn
     1021 ngtancgnat ttccanagat gtggnccg
//
