LOCUS       sequence-183711-         959 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of E. coli LF82_781 CDS fused to mEmerald.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 959)
  AUTHORS   Collins A, Huett A
  TITLE     A multi-phenotypic imaging screen to identify bacterial effectors by
            exogenous expression in a HeLa cell line.
  JOURNAL   Sci Data. 2018 May 15;5:180081. doi: 10.1038/sdata.2018.81.
  PUBMED    29762554
REFERENCE   2  (bases 1 to 959)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Data.";
            date: "2018-05-15"; volume: "5:180081. doi"; pages: " 
            10.1038/sdata.2018.81"
COMMENT     Sequence Label: pCMV_mEm_4GS_LF82_781
FEATURES             Location/Qualifiers
     source          1..959
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    260..394
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(284..303)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     338..357
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(505..521)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(505..521)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(518..540)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    529..545
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(553..559,560..577,578..583))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    598..619
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(736..753)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tcggcatgga cgagctgtac aagcgaattg gtggaggggg cggttccggt ggcggaggtt
       61 cccgaattcg tcgacggaga tctgatatgg caacttatta cagccattgg cggcacgcgt
      121 tgctaattca cgatggtgat tttatttcca cgcaaacggt ttcgtcagcg catcagattc
      181 tttataatga cgcccgtttc ccccccttgg gtacaccgaa agcttgaatc ggtaccctcg
      241 agaagcttgc ggccgcgggg atccagacat gataagatac attgatgagt ttggacaaac
      301 cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt
      361 atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca ttcattttat
      421 gtttcaggtt cagggggagg tgtgggaggt tttttcggat cctctagagt cgatctgcag
      481 gcatgctagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
      541 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
      601 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
      661 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
      721 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
      781 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcnna
      841 aagaacatgt gagcaaaang ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
      901 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtca
//
