LOCUS       sequence-183712-         902 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of E. coli LF82_802 CDS fused to mEmerald.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 902)
  AUTHORS   Collins A, Huett A
  TITLE     A multi-phenotypic imaging screen to identify bacterial effectors by
            exogenous expression in a HeLa cell line.
  JOURNAL   Sci Data. 2018 May 15;5:180081. doi: 10.1038/sdata.2018.81.
  PUBMED    29762554
REFERENCE   2  (bases 1 to 902)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Data.";
            date: "2018-05-15"; volume: "5:180081. doi"; pages: " 
            10.1038/sdata.2018.81"
COMMENT     Sequence Label: pCMV_mEm_4GS_LF82_802
FEATURES             Location/Qualifiers
     source          1..902
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    640..774
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(664..683)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     718..737
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(884..900)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(884..900)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 ttccggtggc ggaggttccc gaattcgtcg acggagatct gatatgccac taacaatttt
       61 tcctgattat caaaaggctg acctaactat gacatttgac cacgtggttg ctttaactac
      121 agctctggga gctgtaattg cagcgtttgc agccatgatt tcagctcacg cgtcaaaaaa
      181 atctacagaa tcagctaggg agacaatgcg ctctaatgag catattgcag agaatgaccg
      241 acgtattcga ctcctagaag aacgtatgaa ggtgtggaat gcttttatag tgctacgtaa
      301 cgtttttacg gtatgtcatg tggtgaacgc gaagactctt gaatctactg ttgatgcaat
      361 gcaaccagcg atatttctgt ttgatcagga ggtttctatt tatctaaaca tattatctgc
      421 tcaattaaga tgtcatttgc aaaagcataa gcggttacag aaccctgctg cgatcttggg
      481 tgttgatgga ttagaaaatg aggccagaga ggaattgatt cgccaaaaag aagaattaga
      541 gatggaaatt aacacaagat tcgacgaggg gaaacctatt ttcataaggc acatgagcct
      601 tatgtgaatc ggtaccctcg agaagcttgc ggccgcgggg atccagacat gataagatac
      661 attgatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa
      721 atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac
      781 aacaattgca ttcattttat gtttcagttc acggggaggt gtgggaggtt ttttcggatc
      841 ctctagagtc gatctgcagg catgctagct tggcgtaatc atggtcatag ctgtttcctg
      901 tg
//
