LOCUS       sequence-183713-         981 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of E. coli LF82_790 CDS fused to mEmerald.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 981)
  AUTHORS   Collins A, Huett A
  TITLE     A multi-phenotypic imaging screen to identify bacterial effectors by
            exogenous expression in a HeLa cell line.
  JOURNAL   Sci Data. 2018 May 15;5:180081. doi: 10.1038/sdata.2018.81.
  PUBMED    29762554
REFERENCE   2  (bases 1 to 981)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Data.";
            date: "2018-05-15"; volume: "5:180081. doi"; pages: " 
            10.1038/sdata.2018.81"
COMMENT     Sequence Label: pCMV_mEm_4GS_LF82_790
FEATURES             Location/Qualifiers
     source          1..981
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    381..515
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(405..424)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     459..478
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(626..642)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(626..642)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(639..661)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    650..666
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(674..680,681..698,699..704))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    719..740
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(857..874)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ctcggcatgg acgagctgta cnagcgaatt ggtggagggg gcggttccgg tggcggaggt
       61 tcccgaattc gtcgacggag atctgatatg ggtaaaaaag acagtaatca ccagattatt
      121 tatcggggcc aggtgctgga acggtttacc cctggcggct gggtcttctt tcaacgccca
      181 aaggagtgtg gcggaggttt ttggttgggc cgcacctatg aagactgctt ctggcttgag
      241 ctggaattcc ccgtttcgct atatgacggc ctggagtttt tgatggaagt caccagggta
      301 gagcagagaa gtgatgaggt tgacgcgaat tattccctgt ttgattgaat cggtaccctc
      361 gagaagcttg cggccgcggg gatccagaca tgataagata cattgatgag tttggacaaa
      421 ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt
      481 tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta
      541 tgtttcaggt tcagggggag gtgtgggagg ttttttcgga tcctctagag tcgatctgca
      601 ggcatgctag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
      661 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
      721 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
      781 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
      841 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
      901 cggtatcagc tcactcaaan ggcggtaata cggntnntcc acagaatcng ggataacgca
      961 ggaaanaaca tgtgagcaaa a
//
