LOCUS       sequence-183717-         923 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of E. coli LF82_811 CDS fused to mEmerald.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 923)
  AUTHORS   Collins A, Huett A
  TITLE     A multi-phenotypic imaging screen to identify bacterial effectors by
            exogenous expression in a HeLa cell line.
  JOURNAL   Sci Data. 2018 May 15;5:180081. doi: 10.1038/sdata.2018.81.
  PUBMED    29762554
REFERENCE   2  (bases 1 to 923)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Data.";
            date: "2018-05-15"; volume: "5:180081. doi"; pages: " 
            10.1038/sdata.2018.81"
COMMENT     Sequence Label: pCMV_mEm_4GS_LF82_811
FEATURES             Location/Qualifiers
     source          1..923
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    483..617
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(507..526)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     561..580
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(728..744)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(728..744)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(741..763)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    752..768
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(776..782,783..800,801..806))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    821..842
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 ctcggcatgg acgagctgta cnagcgaatt ggtggagggg gcggttccgg tggcggaggt
       61 tcccgaattc gtcgacggag atctgatatg gctactacac cattctatta tgccttttca
      121 gcgatatttg atatcgtagc agtattaaat accgaggaaa taatcatgat atcaggtctt
      181 aatggtggaa atttcatcct tagctcgatg gctaaggaaa gagtcaaatc aggtttactt
      241 gatagagcaa caatagaagg ttctaaaggc gagtataccg tgatttttga tggttcagag
      301 tgggaaggta agccattagt cttaagtagc tccagacagc cttatgaagc acgagtattc
      361 aaaagcatag atggtgcagc tgcagaacta ctacgcatag gcttaagcga agcttcaatc
      421 aaaaacacca ccaaatcgaa caataaatga atcggtaccc tcgagaagct tgcggccgcg
      481 gggatccaga catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt
      541 gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa
      601 gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg
      661 aggtgtggga ggttttttcg gatcctctag agtcgatctg caggcatgct agcttggcgt
      721 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
      781 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
      841 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
      901 aatgaatcgg ccaacgcgcg ggg
//
