LOCUS       sequence-183722-         999 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of E. coli LF82_280 CDS fused to mEmerald.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 999)
  AUTHORS   Collins A, Huett A
  TITLE     A multi-phenotypic imaging screen to identify bacterial effectors by
            exogenous expression in a HeLa cell line.
  JOURNAL   Sci Data. 2018 May 15;5:180081. doi: 10.1038/sdata.2018.81.
  PUBMED    29762554
REFERENCE   2  (bases 1 to 999)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Data.";
            date: "2018-05-15"; volume: "5:180081. doi"; pages: " 
            10.1038/sdata.2018.81"
COMMENT     Sequence Label: pCMV_mEm_4GS_LF82_280
FEATURES             Location/Qualifiers
     source          1..999
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ctcggcatgg acgagctgta caagcgaatt ggtggagggg gcggttccgg tggcggaggt
       61 tcccgaattc gtcgacggag atctgatatg agcatgaata tcgcacaaac accatttact
      121 gataaatcgt ttaatcctcg tcgccttgag gaggcaagag aggcaaaagg gttgacgatg
      181 gctgaacttg ccagagtgct caatatttca agacaagcta tttcgtcatt tgagaagggg
      241 ttgaagtctc cctctgctga tacactttct gcaatagcca aggtgttagg atttccagaa
      301 cgatttttct tagcctccag tgcttctcca tctctggagg gggcgattca ttttagaagt
      361 cgttcaactg cgaccaaaaa ggctcgagtt acagggaaaa cacgtgggcg ttgggcggca
      421 ttaatattgg atgaatgtct aaaatatgct caattaccag atgtggttct gcctgaattc
      481 gatattattg attttgaggt tctgtctcta tctgatattg aggatatgtc cacccaatta
      541 aggcgttttt gggggttagg tgatggtcca atactcaatt taactagact tgttgaaaat
      601 aaaggaatcg tggtttctca tttaccttct ggtgagaaag ttgatgcctt ttcgttctgg
      661 cataatgggc ggcctttaat tatgttagat agctcgaaga cagctgttcg catgcgattt
      721 agtcttgctc atgaacttgg ccatttaatt atgcataggg cagtggagga tgactacctg
      781 aatgataaag aattattcga cctggtggag ttgcangccg attattttgc ctccagcttt
      841 ttaatgccag ccaccacttt cgggcgtgaa ttttatagtc caaatttatc agcgttagaa
      901 cgattaaaac tacgttggat tacatctatc ggtagtattg ccatgaggag tcatagcctt
      961 aagttaatca gcgatnatca gaaaagctat atatttnag
//
