LOCUS       sequence-183723-        1024 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of E. coli LF82_805 CDS fused to mEmerald.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1024)
  AUTHORS   Collins A, Huett A
  TITLE     A multi-phenotypic imaging screen to identify bacterial effectors by
            exogenous expression in a HeLa cell line.
  JOURNAL   Sci Data. 2018 May 15;5:180081. doi: 10.1038/sdata.2018.81.
  PUBMED    29762554
REFERENCE   2  (bases 1 to 1024)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Data.";
            date: "2018-05-15"; volume: "5:180081. doi"; pages: " 
            10.1038/sdata.2018.81"
COMMENT     Sequence Label: pCMV_mEm_4GS_LF82_805
FEATURES             Location/Qualifiers
     source          1..1024
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    450..584
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(474..493)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     528..547
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(695..711)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(695..711)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(708..730)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    719..735
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(743..749,750..767,768..773))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    788..809
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(926..943)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ctcggcatgg ncgagctgta caagcgaatt ggtggagggg gcggttccgg tggcggaggt
       61 tcccgaattc gtcgacggag atctgatatg tctgaaaaaa ctaaacttga gatattttcc
      121 gttgaataca gaaaaaatat ttttgattta tataaagata catttgtttg cgtagctatt
      181 gctgttttgg gcgtttactt gattaaaaat gattatcaat ttattggatg gttaacatat
      241 attgttgctg gtgcattatc catactggca tcaactacta ccattaaaat gattggagaa
      301 aagcgaaaat cagaggaaac aagtaaatca ggattcttgc tgttgttatt cttcagcctg
      361 ttttccataa taattctagt tgctggttta gttaaagggg taaaggatgt tctgtgaatc
      421 ggtaccctcg agaagcttgc ggccgcgggg atccagacat gataagatac attgatgagt
      481 ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg
      541 ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca
      601 ttcattttat gtttcaggtt cagggggagg tgtgggaggt tttttcggat cctctagagt
      661 cgatctgcag gcatgctagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
      721 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
      781 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
      841 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
      901 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
      961 tgcggcgagc ggtatcagct cactcaangg cggtanacgn ttatccacag antcnnggnt
     1021 acgc
//
