LOCUS       sequence-183724-        1008 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of E. coli LF82_313 CDS fused to mEmerald.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1008)
  AUTHORS   Collins A, Huett A
  TITLE     A multi-phenotypic imaging screen to identify bacterial effectors by
            exogenous expression in a HeLa cell line.
  JOURNAL   Sci Data. 2018 May 15;5:180081. doi: 10.1038/sdata.2018.81.
  PUBMED    29762554
REFERENCE   2  (bases 1 to 1008)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Data.";
            date: "2018-05-15"; volume: "5:180081. doi"; pages: " 
            10.1038/sdata.2018.81"
COMMENT     Sequence Label: pCMV_mEm_4GS_LF82_313
FEATURES             Location/Qualifiers
     source          1..1008
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    552..686
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(576..595)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     630..649
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(797..813)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(797..813)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(810..832)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    821..837
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(845..851,852..869,870..875))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    890..911
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 ctcggcatgg acganctgta cnagcgaatt ggtggagggg gcggttccgg tggcggaggt
       61 tcccgaattc gtcgacggag atctgatatg gtcgccgttt taccggcaat gccgcctttg
      121 aaaactgccg gatctgtcct tcgatggatt aaggctttct tcatacttta ttacgctaag
      181 gaaatcacaa tgaatctgat gaataattgt gcatcgcctg ccgtagatgt tatattaaaa
      241 tataatattc ggaggtgctc tatgtataca actcgcctga aaaaggtcgg cggatccatc
      301 atgctggcgg ttccccccgc cgtgctgaaa acgctggagc tgtcgacgga cagcgaagtg
      361 ggtatgacca ttgataatgg ctgcctgatt attgaacccc agaaacggcc ccgttattcg
      421 cttgaggagt tgttggcgca gtgcgatccg cacgcagaaa tgagcgaaga ggatcgggaa
      481 tggattgatg cgcctgcggt gggcaaggag atcctgtgaa tcggtaccct cgagaagctt
      541 gcggccgcgg ggatccagac atgataagat acattgatga gtttggacaa accacaacta
      601 gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa
      661 ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg
      721 ttcaggggga ggtgtgggag gttttttcgg atcctctaga gtcgatctgc aggcatgcta
      781 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
      841 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
      901 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
      961 agctgcattn atgnatcggn ccaacgcgcg ggganaggcg gtttgcgt
//
