LOCUS       sequence-183727-         940 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of E. coli LF82_031 CDS fused to mEmerald.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 940)
  AUTHORS   Collins A, Huett A
  TITLE     A multi-phenotypic imaging screen to identify bacterial effectors by
            exogenous expression in a HeLa cell line.
  JOURNAL   Sci Data. 2018 May 15;5:180081. doi: 10.1038/sdata.2018.81.
  PUBMED    29762554
REFERENCE   2  (bases 1 to 940)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Data.";
            date: "2018-05-15"; volume: "5:180081. doi"; pages: " 
            10.1038/sdata.2018.81"
COMMENT     Sequence Label: pCMV_mEm_4GS_LF82_031
FEATURES             Location/Qualifiers
     source          1..940
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    786..920
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(810..829)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     864..883
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 ctcggcatgg acgagctgta caagcgaatt ggtggagggg gcggttccgg tggcggaggt
       61 tcccgaattc gtcgacggag atctgatatg cgtaaaaaat acatttctgt tagagttttt
      121 tttatgtgtc ttttaatact aacagtagct ggttgctatg gaaaaggaaa tgaaaactta
      181 atattgcctt ttgattcaac tcaatctttt caattggaat gttattatca atatattaat
      241 aatatgcatg atattacaga gataaagtta tcggaagtgc ataataaaca aactaaggca
      301 tttagtcagc aagaaaccaa gattcctgtg aaaggtgaga attttctctc tccatatgtg
      361 gatattaatg atcgctatta tgaattaggc cattttgaat atgaggataa tacatataaa
      421 ttgatagttt ataacaagat aggggagtct gatacactat tgcttaatgt acagataaat
      481 agctataatg caacggagaa tttagtggat gctttattac ttggcagctt ttttggttat
      541 gaagatatag tccgcttttc tgattttatc attcacccag actattcaat aagtattgat
      601 aattatgtcg tatatgcata cgaagaaagc aaggatggtc tttcaactag aatagctaaa
      661 gatccaatcc ctcatattta tttaaaagag caatataaaa tagaaaatgg tcagttcaag
      721 ttgacatcca gaatggaaat attaaaaaat tgaatcggta ccctcgagaa gcttgcggcc
      781 gcggggatcc agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc
      841 agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta
      901 taagctgcaa taaacaagtt aacaacaaca attgcattca
//
