LOCUS       sequence-183733-         916 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of E. coli LF82_124 CDS fused to mEmerald.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 916)
  AUTHORS   Collins A, Huett A
  TITLE     A multi-phenotypic imaging screen to identify bacterial effectors by
            exogenous expression in a HeLa cell line.
  JOURNAL   Sci Data. 2018 May 15;5:180081. doi: 10.1038/sdata.2018.81.
  PUBMED    29762554
REFERENCE   2  (bases 1 to 916)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Data.";
            date: "2018-05-15"; volume: "5:180081. doi"; pages: " 
            10.1038/sdata.2018.81"
COMMENT     Sequence Label: pCMV_mEm_4GS_LF82_124
FEATURES             Location/Qualifiers
     source          1..916
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    264..398
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(288..307)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     342..361
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(509..525)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(509..525)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(522..544)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    533..549
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(557..563,564..581,582..587))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    602..623
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(740..757)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ctcggcatgg acgagctgta caagcgaatt ggtggagggg gcggttccgg tggcggaggt
       61 tcccgaattc gtcgacggag atctgatatg atagcgataa ttttactcat catccttcac
      121 atctggctct gtagacaggg tggtgatcac ttctggagta aatccagatt aaacatctca
      181 ttgctgatgc ttgatattga gcatctggcg cgcagtaagg ggctgcgttg aatcggtacc
      241 ctcgagaagc ttgcggccgc ggggatccag acatgataag atacattgat gagtttggac
      301 aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg
      361 ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt
      421 ttatgtttca ggttcagggg gaggtgtggg aggttttttc ggatcctcta gagtcgatct
      481 gcaggcatgc tagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
      541 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
      601 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
      661 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
      721 ttgggcgctc ntccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
      781 gagcggtatc agctcactcn aaggcggtaa tacggttatc cacagaatcn ngggataacg
      841 cnnnaagaac atgtgancaa aangccngca aaaggccagg aaccgtaaaa aggccgcgtt
      901 gctggcgttt ttccat
//
