LOCUS       sequence-183737-         943 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of E. coli LF82_189 CDS fused to mEmerald.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Collins A, Huett A
  TITLE     A multi-phenotypic imaging screen to identify bacterial effectors by
            exogenous expression in a HeLa cell line.
  JOURNAL   Sci Data. 2018 May 15;5:180081. doi: 10.1038/sdata.2018.81.
  PUBMED    29762554
REFERENCE   2  (bases 1 to 943)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Data.";
            date: "2018-05-15"; volume: "5:180081. doi"; pages: " 
            10.1038/sdata.2018.81"
COMMENT     Sequence Label: pCMV_mEm_4GS_LF82_189
FEATURES             Location/Qualifiers
     source          1..943
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(914..933)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tcggcatggn cgagctgtac aagcgaattg gtggaggggg cggttccggt ggcggaggtt
       61 cccgaattcg tcgacggaga tctgatatga ttgatacaat gaaaacctta agtgggaaag
      121 agtttaggaa attatttaat cgttatgaga caggattcta tctaagtgag tttgaatact
      181 taaaggactt tgatcctttg tttcctgaat gcgtagggaa agaattaaag ttgggttata
      241 aacgaaaagt tgatgtatgg tgcgaagagg atgctgcgct atatgcagaa tttatagcta
      301 caaaagaaag gctgaaaaga ggccggttaa tcctaaaaat aaaccaagtg tggtcgcgtg
      361 tatatcctgc ttatatttat gcaattgaag aaagaataaa tgtcagggca aaggggagag
      421 agtcatttaa gtatgaaact gtgcaagaaa aacaatgtaa actttccata atgttagatg
      481 gtctgaaaga ccttgaatgt gaaatagcta tgatttcaag tgagtttgaa attgaaaaac
      541 actctatgca ctatttttat atgatttttg atattgaacg ctatataaat gactgcagtt
      601 actttgtttt ttctgctgct aaagttgtta aaggcgtgat tcaatgcatt ggttacattg
      661 acaaaaataa caaagcaagt tgcgatgaat tttattcagg cagctatgaa tgcttggatg
      721 atgatcagga aacactgaac tatttaaatg aatcatgcgc agagggtata gacgctgata
      781 aagatctcat caggaagaat gagttattag ccttatccct gaaggaattg tatcaacttc
      841 tcgtttcaaa aaaatgaatc ggtaccctcg agaagcttgc ggccgcgggg atccagacat
      901 gataagatac attgatgagt ttggacaaac cacaactaga atg
//
