LOCUS       sequence-183740-        1017 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of E. coli LF82_681 CDS fused to mEmerald.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1017)
  AUTHORS   Collins A, Huett A
  TITLE     A multi-phenotypic imaging screen to identify bacterial effectors by
            exogenous expression in a HeLa cell line.
  JOURNAL   Sci Data. 2018 May 15;5:180081. doi: 10.1038/sdata.2018.81.
  PUBMED    29762554
REFERENCE   2  (bases 1 to 1017)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Data.";
            date: "2018-05-15"; volume: "5:180081. doi"; pages: " 
            10.1038/sdata.2018.81"
COMMENT     Sequence Label: pCMV_mEm_4GS_LF82_681
FEATURES             Location/Qualifiers
     source          1..1017
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    339..473
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(363..382)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     417..436
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(584..600)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(584..600)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(597..619)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    608..624
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(632..638,639..656,657..662))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    677..698
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(815..832)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ctcngnatgg ncganctgta caagcgaatt ggtggagggg gcggttccgg tggcggaggt
       61 tcccgaattc gtcgacggag atctgatatg aagcagttgg agcaagtgca ggctgcgctg
      121 gaacgcgccc gtgttgtcgc cgttcaggat gggcagcacg gacgcttcta tttcgattac
      181 ctacgggcaa cgcaggatat caaagcgatt cgccagggga ttggtatcta cctggaacct
      241 tcccgcgctc agccggtgcc ttcggcctct ctcaccggtc agtaccggat ggagcgtcgc
      301 ccgtgaatcg gtaccctcga gaagcttgcg gccgcgggga tccagacatg ataagataca
      361 ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa
      421 tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca
      481 acaattgcat tcattttatg tttcaggttc agggggaggt gtgggaggtt ttttcggatc
      541 ctctagagtc gatctgcagg catgctagct tggcgtaatc atggtcatag ctgtttcctg
      601 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
      661 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
      721 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
      781 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
      841 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg gttatccaca
      901 gaatcngggg anaacgcagg aaagaacatg tgagcaaaag gncagcaaaa ggccaggaac
      961 cgtaaaaagg ccgcgttgct gggcgttttn cataggctcc nnccccngac gagcatc
//
