LOCUS       sequence-183741-         941 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of E. coli LF82_249 CDS fused to mEmerald.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 941)
  AUTHORS   Collins A, Huett A
  TITLE     A multi-phenotypic imaging screen to identify bacterial effectors by
            exogenous expression in a HeLa cell line.
  JOURNAL   Sci Data. 2018 May 15;5:180081. doi: 10.1038/sdata.2018.81.
  PUBMED    29762554
REFERENCE   2  (bases 1 to 941)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Data.";
            date: "2018-05-15"; volume: "5:180081. doi"; pages: " 
            10.1038/sdata.2018.81"
COMMENT     Sequence Label: pCMV_mEm_4GS_LF82_249
FEATURES             Location/Qualifiers
     source          1..941
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(906..925)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 ctcggcatgg acgagctgta caagcgaatt ggtggagggg gcggttccgg tggcggaggt
       61 tcccgaattc gtcgacggag atctgatatg aacaatacca cgcccattaa gatattaacg
      121 gcagcggtac tgctaacctt tggttttgcg cttaccgcaa atgctgcgct catcaataaa
      181 ggagtcaccg aaatgaatca tgaatatacg gtttcagata cgctgtcagc tcgccagcag
      241 gccattccgt tgattgccgc atcaatggcc agcagtcaga tggataagct gaatgcagcg
      301 cttaatcaag gacttgatgc aggacttacg ataaacgaag ctaaggaaat tctcatccag
      361 ctctatgcct atacgggatt tccccgcagc ctgaatgcac tgaatgagct gatgaaagtc
      421 gttgaaacac gtaaacaacg tggcatcgaa gacattgaag gtaaagcgcc gatcgccccg
      481 attcccgtcg gggatgagct tcggcgtgtc ggtaccgcaa accagacgaa aatctcaggc
      541 gcgcctgtgc aggggccact gtttgatttt gcgccaacaa taaatcaatt cctgcaaaca
      601 catctgttcg gcgatatttt cgctcgcgat aacctcgact ggcaaagccg tgaactggca
      661 acagttggag cattagccgc tacgccaggc gttgaagccc agttactgtc acatacgcga
      721 gcaagtctgc gagtcggtct gacggcagcg cagttacgac agttagcaca ggttctgcgt
      781 gaacatggcg acaatgatgc agctacaaga gctgatgagg cactgcaaaa agcgcttgag
      841 aacacatgaa tcggtaccct cgagaagctt gcggccgcgg ggatccagac atgataagat
      901 acattgatga gtttggacaa accacaacta gaatgcagtg a
//
