LOCUS       sequence-183744-         950 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of E. coli LF82_259 CDS fused to mEmerald.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 950)
  AUTHORS   Collins A, Huett A
  TITLE     A multi-phenotypic imaging screen to identify bacterial effectors by
            exogenous expression in a HeLa cell line.
  JOURNAL   Sci Data. 2018 May 15;5:180081. doi: 10.1038/sdata.2018.81.
  PUBMED    29762554
REFERENCE   2  (bases 1 to 950)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Data.";
            date: "2018-05-15"; volume: "5:180081. doi"; pages: " 
            10.1038/sdata.2018.81"
COMMENT     Sequence Label: pCMV_mEm_4GS_LF82_259
FEATURES             Location/Qualifiers
     source          1..950
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    414..548
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(438..457)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     492..511
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(660..676)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(660..676)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(673..695)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    684..700
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(708..714,715..732,733..738))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    753..774
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(891..908)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ctcggcatgg acgagctgta caagcgaatt ggtggagggg gcggttccgg tggcggaggt
       61 tcccgaattc gtcgacggag atctgatatg gatataacac ctttccttca tgcgctttgt
      121 gctgtggctg cgcagctact gattggtctt tttaccggga actgggctta cggggcgata
      181 gccggttgta cgttcttcat tgcgcgtgaa cacacccagg cagaatatcg ctggattgaa
      241 atgttcgggc atggcaagcg aatgaatatg ccgtggtggg gcggttttga tccgcgcgcg
      301 tgggatgtgg caagcctgat ggattttgct gtgccggtgg tggcgtgtct gctggtctgg
      361 ctgttggtta atcgtgggtg aatcggtacc ctcgagaagc ttgcggccgc ggggatccag
      421 acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat
      481 gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata
      541 aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg
      601 gaggtttttt cggatcctct agagtcgatc tgcaggcatg ctagcttggc gtaatcatgg
      661 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
      721 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
      781 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
      841 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
      901 gactcgctgc gctcggtcgt tcggctgcgg cgagcgntat cagctcactc
//
