LOCUS       sequence-18375-e         783 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 783)
  AUTHORS   Colwill K, Wells CD, Elder K, Goudreault M, Hersi K, Kulkarni S, 
            Hardy WR, Pawson T, Morin GB
  TITLE     Modification of the Creator recombination system for proteomics 
            applications--improved expression by addition of splice sites.
  JOURNAL   BMC Biotechnol. 2006 Mar 6;6:13. doi: 10.1186/1472-6750-6-13.
  PUBMED    16519801
REFERENCE   2  (bases 1 to 783)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2006-03-6"; volume: "6:13. doi"; pages: " 
            10.1186/1472-6750-6-13"
COMMENT     Sequence Label: V308 pDNR MCS lacZ alpha - Donor
FEATURES             Location/Qualifiers
     source          1..783
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(3..25)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      complement(206..300)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
ORIGIN
        1 gtcgttttac aacgtcgtga ctggggaaaa ccctgggcgt tacccaactt aatcgccttg
       61 cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt
      121 cccaacagtt gcgcagcctg aatggcgaat ggcgttaatt aaccaggtaa gttcctagga
      181 gatcctggtc atgactagtg cttggattct caccaataaa aaacgcccgg cggcaaccga
      241 gcgttctgaa caaatccaga tggagttctg aggtcattac tggatctatc aacaggagtc
      301 caagcgagct cgatatcaaa ttacgccccg ccctgccact catcgcagta ctgttgtaat
      361 tcattaagca ttctgccgac atggaagcca tcacaaacgg catgatgaac ctgaatcgcc
      421 agcggcatca gcaccttgtc gccttgcgta taatatttgc ccatggtgaa aacgggggcg
      481 aagaagttgt ccatattggc cacgtttaaa tcaaaactgg tgaaactcac ccagggattg
      541 gctgagacga aaaacatatt ctcaataaac cctttaggga aataggccag gttttcaccg
      601 taacacgcca catcttgcga atatatgtgt agaaactgcc ggaaatcgtc gtggtattca
      661 ctccagagcg atgaaaacgt ttcagtttgc tcatggaaaa cggtgtaaca agggtgaaca
      721 ctatcccata tcaccagctc accgtctttc attgccatac gaaattccgg atgagcattc
      781 atc
//
