LOCUS       sequence-183754-        1063 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of E. coli LF82_436 CDS fused to mEmerald.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1063)
  AUTHORS   Collins A, Huett A
  TITLE     A multi-phenotypic imaging screen to identify bacterial effectors by
            exogenous expression in a HeLa cell line.
  JOURNAL   Sci Data. 2018 May 15;5:180081. doi: 10.1038/sdata.2018.81.
  PUBMED    29762554
REFERENCE   2  (bases 1 to 1063)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Data.";
            date: "2018-05-15"; volume: "5:180081. doi"; pages: " 
            10.1038/sdata.2018.81"
COMMENT     Sequence Label: pCMV_mEm_4GS_LF82_436
FEATURES             Location/Qualifiers
     source          1..1063
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    393..527
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(417..436)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     471..490
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(638..654)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(638..654)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(651..673)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    662..678
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(686..692,693..710,711..716))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    731..752
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(869..886)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ctcggcatgg acgagctgta caagcgaatt ggtggagggg gcggttccgg tggcggaggt
       61 tcccgaattc gtcgacggag atctgatatg gaaagtgata tgggaaaaaa tgtggtagta
      121 ctcaatgacg caacttctca cggtggcaaa gtctcttcgg cctcttcaac ctttgatatt
      181 cagggaaaaa aggctgcact gctgaatgat accgtcactt gcccgaagca tggtactaac
      241 ccaattgtgg agtgtgatac cagttatgag gagggtggac gtgggattgt tgtgcatggc
      301 tgtaaaagcg cctgcggctc ggtggtgtat gccagtctgc cggatgtgga gataggctga
      361 atcggtaccc tcgagaagct tgcggccgcg gggatccaga catgataaga tacattgatg
      421 agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg
      481 atgctattgc tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt
      541 gcattcattt tatgtttcag gttcaggggg aggtgtggga ggttttttcg gatcctctag
      601 agtcgatctg caggcatgct agcttggcgt aatcatggtc atagctgttt cctgtgtgaa
      661 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
      721 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
      781 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
      841 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
      901 ggctgcngcg agcggtatca gctcactcaa aggcggtaat acgnttatcc acagaatcag
      961 gggataacgc aggaaagaac atgtgagcaa agccagcaaa aggccaggaa ccgtaaaagg
     1021 cgcntgctgg cgtttttcat aggctcgccc ccngacnagc atc
//
