LOCUS       sequence-183757-         827 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of E. coli LF82_499 CDS fused to mEmerald.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 827)
  AUTHORS   Collins A, Huett A
  TITLE     A multi-phenotypic imaging screen to identify bacterial effectors by
            exogenous expression in a HeLa cell line.
  JOURNAL   Sci Data. 2018 May 15;5:180081. doi: 10.1038/sdata.2018.81.
  PUBMED    29762554
REFERENCE   2  (bases 1 to 827)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Data.";
            date: "2018-05-15"; volume: "5:180081. doi"; pages: " 
            10.1038/sdata.2018.81"
COMMENT     Sequence Label: pCMV_mEm_4GS_LF82_499
FEATURES             Location/Qualifiers
     source          1..827
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    596..730
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(620..639)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     674..693
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 gcatggncga nctgtacaag cgaattggtg gagggggcgg ttccggtggc ggaggttccc
       61 gaattcgtcg acggagatct gatatgtcaa atatcatata cttatcaatt aaagggaaaa
      121 tacaaggttt gatctctgag ggttgtggtt cgtatgcatc tattggtaat aaatatcaaa
      181 taaatcatgt ggatgaaatt tttgttttac agttcgacca ttctttaagt cgagagtata
      241 atgtagtgca tcaccccata aaattttaca agccaataga taaatcctcc cctttattaa
      301 atgccgctct ttctgaaaat gaagagttat cagctgtatt taatttttat agagttaatt
      361 ctggcggttg tattgaaagg ttttacacaa ttgaattgca aggggcatat ttatcgaata
      421 ttctgacttg ttatccacac tcaattacac atgcgggtaa tcaaccggaa gaggtcattg
      481 ttcttaatta taaaaatata acatggaagc accatatcgc gagtacggaa tcatatagct
      541 gttgggaaga aaggattttt tgaatcggta ccctcgagaa gcttgcggcc gcggggatcc
      601 agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa
      661 atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa
      721 taaacaagtt aacaacaaca attgcattca ttttatgttt caggntcang gggaggtgtg
      781 ggaggttttt tcggatcctc tagagtcgat ctgcaggcat gctagct
//
