LOCUS       sequence-183758-        1031 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of E. coli LF82_534 CDS fused to mEmerald.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1031)
  AUTHORS   Collins A, Huett A
  TITLE     A multi-phenotypic imaging screen to identify bacterial effectors by
            exogenous expression in a HeLa cell line.
  JOURNAL   Sci Data. 2018 May 15;5:180081. doi: 10.1038/sdata.2018.81.
  PUBMED    29762554
REFERENCE   2  (bases 1 to 1031)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Data.";
            date: "2018-05-15"; volume: "5:180081. doi"; pages: " 
            10.1038/sdata.2018.81"
COMMENT     Sequence Label: pCMV_mEm_4GS_LF82_534
FEATURES             Location/Qualifiers
     source          1..1031
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(891..910)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     945..964
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 ctcggcatgg acgagctgta caagcgaatt ggtggagggg gcggttccgg tggcggaggt
       61 tcccgaattc gtcgacggag atctgatatg aaatcattga gtaagtcgtc acaaataccg
      121 ctctatcaac aagtggtgga gtggataaga gaaagtattt ataccgggga tctggtggaa
      181 gacgatcgca ttccttcgga ataccagatt atggatatgc tggaagtgag ccggggaacc
      241 gttaaaaaag cggtcgccca actggtaaaa gaaggcgtgt tgatacaggt ccaggggaag
      301 ggaacatttg tcaaaaaaga gaacgtggca tatccgttag gtgaaggatt attgtcattc
      361 gcggaatcgc tggaaagcca gaaaatacat tttaccactg aagttattac gtcacggatt
      421 gaaccggcta atcgttatgt ggcagagaaa ttaagaataa cgcccggtca ggatattctt
      481 taccttgagc gtttacgttc aattggtgat gaaaaagcga tgctgataga gaaccgtatc
      541 aatattgagc tatgccccgg catcgtggaa atcgatttta atcaacacaa tttatttcca
      601 acaatagaaa gtttgtcgca aagaaaaatt cgttactcgg aaagtcgcta tgccgcgcga
      661 ttaattggta atgaacgcgg tcatttttta gatatcagtg aagatgcacc cgttttgcat
      721 ctggagcagt tagtcttttt ctcccgagga ttacccgttg agtttggcaa cgtctggtta
      781 aaaggaaata aatactatct tggcaccgtg ctgcaacggc gggaagtgag ttgaatcggt
      841 accctcgaga agcttgcggc cgcggggatc cagacatgat aagatacatt gatgagtttg
      901 gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta
      961 ttgctttatt tgtaaccatt ataagctgca naannagttn acaacaacan ngcattcatt
     1021 ttatgtttcn g
//
