LOCUS       sequence-183760-        1043 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of E. coli LF82_0557 CDS fused to mEmerald.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1043)
  AUTHORS   Collins A, Huett A
  TITLE     A multi-phenotypic imaging screen to identify bacterial effectors by
            exogenous expression in a HeLa cell line.
  JOURNAL   Sci Data. 2018 May 15;5:180081. doi: 10.1038/sdata.2018.81.
  PUBMED    29762554
REFERENCE   2  (bases 1 to 1043)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Data.";
            date: "2018-05-15"; volume: "5:180081. doi"; pages: " 
            10.1038/sdata.2018.81"
COMMENT     Sequence Label: pCMV_mEm_4GS_LF82_0557
FEATURES             Location/Qualifiers
     source          1..1043
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    474..608
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(498..517)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     552..571
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(719..735)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(719..735)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(732..754)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    743..759
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(767..773,774..791,792..797))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    812..833
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(950..967)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ctcggcatgg acgagctgta caagcgaatt ggtggagggg gcggttccgg tggcggaggt
       61 tcccgaattc gtcgacggag atctgatatg aacaggaaag aatatgctat gaacccttat
      121 atttatcttg gtggcgcaat acttgcagag gtcattggta caaccttaat gaagttttca
      181 gaggggttta cacggttatg gccctctgtt ggcacaatta tttgctattg tgcatcattc
      241 tggttattag ctcagacgct ggcttatata cctacaggga ttgcttatgc tatctggtca
      301 ggagtcggta tcgttctgat tagtttgctt tcctggggat tttttggtca acgactggac
      361 ctgccagcta tcataggcat gatgttgatt tgtgccggtg tgttagtcat taacttgttg
      421 tcacgaagtg caccgcattg aatcggtacc ctcgagaagc ttgcggccgc ggggatccag
      481 acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat
      541 gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata
      601 aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg
      661 aggttttttc ggatcctcta gagtcgatct gcaggcatgc tagcttggcg taatcatggt
      721 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
      781 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
      841 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
      901 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
      961 actcgctgcg ctcggtcgtt cngctgcggc gagcggtanc agctcactca aaggcggtat
     1021 acgnttatcc acagaatcng gga
//
