LOCUS       sequence-183761-         984 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of E. coli LF82_602 CDS fused to mEmerald.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 984)
  AUTHORS   Collins A, Huett A
  TITLE     A multi-phenotypic imaging screen to identify bacterial effectors by
            exogenous expression in a HeLa cell line.
  JOURNAL   Sci Data. 2018 May 15;5:180081. doi: 10.1038/sdata.2018.81.
  PUBMED    29762554
REFERENCE   2  (bases 1 to 984)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Data.";
            date: "2018-05-15"; volume: "5:180081. doi"; pages: " 
            10.1038/sdata.2018.81"
COMMENT     Sequence Label: pCMV_mEm_4GS_LF82_602
FEATURES             Location/Qualifiers
     source          1..984
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    819..953
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(843..862)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     897..916
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 ctcggcatgg ncgagctgta caagcgaatt ggtggagggg gcggttccgg tggcggaggt
       61 tcccgaattc gtcgacggag atctgatatg atctggcggt taatcgcgct cgatttagac
      121 ggcacattgc tgaacagcaa gaagatgatt ttccctgcgt ctgtttcggc actgcaaagc
      181 gcccaacagg caggcgtgaa gattattctc gccaccgggc gttcgcacag cgaagctctg
      241 ccttattacc agcaattgca gttaaccggg ccgatgattt gctgtaacgg cagctatctt
      301 tatcatccgc gccagcggca aatattacgt ccgctgccgc ttgatatcca caaggtggaa
      361 cggctacgac gctggttttc acaacaggca ttacagccac gtttttatac gtatgatgat
      421 tttgttttgc agattagcct gatacatcgc aaggcttcgt tattgcgcca ggttgaggca
      481 tttgcccggc aagaattatc gctggcctgt agctggtcct ggcatcatca actggatatt
      541 acacaggcgg agtgtgagaa aggcaaaagt ctggcctggt atgcacagca gcaaaatatc
      601 gctctggatg aaatcatcgc ctttggcgat aacgataatg atgcggggat gttgcagatg
      661 gtagggaaag gtattgcgat gggcaatggt agctttctgg caaaagccag ctctaactgc
      721 gttatcggtc acaacaacac tgatgcgatt gcggattttc tttatgcgca ggggttcagt
      781 tcatgaatcg gtaccctcga gaagcttgcg gccgcgggga tccagacatg ataagataca
      841 ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa
      901 tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca
      961 acaattgcat tcattttatg tttc
//
