LOCUS       sequence-183764-        1043 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of E. coli LF82_658 CDS fused to mEmerald.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1043)
  AUTHORS   Collins A, Huett A
  TITLE     A multi-phenotypic imaging screen to identify bacterial effectors by
            exogenous expression in a HeLa cell line.
  JOURNAL   Sci Data. 2018 May 15;5:180081. doi: 10.1038/sdata.2018.81.
  PUBMED    29762554
REFERENCE   2  (bases 1 to 1043)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Data.";
            date: "2018-05-15"; volume: "5:180081. doi"; pages: " 
            10.1038/sdata.2018.81"
COMMENT     Sequence Label: pCMV_mEm_4GS_LF82_658
FEATURES             Location/Qualifiers
     source          1..1043
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ctcggcatgg acgagctgta caagcgaatt ggtggagggg gcggttccgg tggcggaggt
       61 tcccgaattc gtcgacggag atctgatatg catgtaattt cagtaatttc tactaaaggt
      121 ggtgaaggta aatccactca ttccgccaac ctggctggct tctgtgccga tgccggtctc
      181 aaaaccctcc tgattgacgg cgactacgcc cagcccacgg tttccagtta ctatcctttg
      241 cgctacgaag cgccatgtgg tttgtacgag ttacttatgc agactgtgga tcttaatcgg
      301 ccggaacaac tgatttccca cactgccatt gataaccttg atctgattat ttctaacgat
      361 ccttatggtc agttaaaaat ggcaatgatg cacgctccgg acgggcgcat cagactacgt
      421 aaccagcttc agcatccatt gtttgcctct tatgacgtga ttatcagtga tacgcaaggc
      481 gcgcgctcaa tcatgttaga gctgatgttg ttggcgacaa cacatacggc actggcggtt
      541 gtgaaaccgg taataccgga cgttcgcgag tttctacggg gaacggtaac gctgatggag
      601 gatctactgc cttaccgtgg atttggtatt accttgccgt caatgaaaat cctggtcaac
      661 tgcatggatt acaccctgct ggctgaagaa gcactgggtg cgttaactac gattattgct
      721 gatgggacgt atagcaaagc ggatattccc cccgtcactc tgctcgaaac ccaaatctac
      781 gatcttgaag tctacaagtt aggccatacg cgggggcaac ccgctcaccg tcttgaatac
      841 cataccgatc gtaaaagttt gccagccgct cagactatgc acgatctggn cntgcgaatt
      901 atttcccgaa tggngcgacc gttttgatgc cgtactgcaa aaccagccga aggggataaa
      961 tgaatcggta ccctcgagaa gcttgcngcc gcgggntccn nacatgataa nannacattg
     1021 atgantttgg annaannaca cta
//
