LOCUS       sequence-183765-        1028 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of E. coli LF82_663 CDS fused to mEmerald.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1028)
  AUTHORS   Collins A, Huett A
  TITLE     A multi-phenotypic imaging screen to identify bacterial effectors by
            exogenous expression in a HeLa cell line.
  JOURNAL   Sci Data. 2018 May 15;5:180081. doi: 10.1038/sdata.2018.81.
  PUBMED    29762554
REFERENCE   2  (bases 1 to 1028)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Data.";
            date: "2018-05-15"; volume: "5:180081. doi"; pages: " 
            10.1038/sdata.2018.81"
COMMENT     Sequence Label: pCMV_mEm_4GS_LF82_663
FEATURES             Location/Qualifiers
     source          1..1028
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    645..779
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(669..688)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     723..742
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(889..905)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(889..905)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(902..924)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    913..929
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 ctcggcatgg acgagctgta caagcgaatt ggtggagggg gcggttccgg tggcggaggt
       61 tcccgaattc gtcgacggag atctgatatg aacggactgt taacgcagat tgttatggaa
      121 ctgaaatccg gcaatatccg ccgttgtgag gcgatggggt taaccctgga agagattcag
      181 gaacttaaca gcttaaccgt tgaagatctg cattatctgg tgaatacgca ggtatcgctt
      241 ctgaccttcc gcatcaacca taccaacctg aacaccatgc tggcgcaggc ccgtcgtgag
      301 cagacccgga cacagcgtat cgacagagcg ttaacgctgg gtggctctat cgagctgatg
      361 cagcattttt tcggcctgac ggcgacggaa gtcagttccc ggcgacgcct ggtcggtgtt
      421 gtgacccggc aggggcgcag ccagatccct acagaagagc aggaactggc ggtgtggcag
      481 cagtggaagg catcgaacgt tgacaacatg gaatcactgg atgcgctgga agccatgatg
      541 ttaattgctg agcagcagga catttcgtta accgcagtct ggacgttaac caaaggatgg
      601 agtgccgcct gaatcggtac cctcgagaag cttgcggccg cggggatcca gacatgataa
      661 gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt
      721 gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta
      781 acaacaacaa ttgcattcat tttatgtttc angttcaggg ggaggtgtgg gaggtttttt
      841 cggatcctct agagtcgatc tgcnncatgc tagcttggng taatcatggt catagctgtt
      901 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataag
      961 tgtaaagcct ggggtgccta angagtgagc taactcacat taattgcgtt gcnctcactg
     1021 nccgcttt
//
