LOCUS       sequence-183768-        1028 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of E. coli LF82_666 CDS fused to mEmerald.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1028)
  AUTHORS   Collins A, Huett A
  TITLE     A multi-phenotypic imaging screen to identify bacterial effectors by
            exogenous expression in a HeLa cell line.
  JOURNAL   Sci Data. 2018 May 15;5:180081. doi: 10.1038/sdata.2018.81.
  PUBMED    29762554
REFERENCE   2  (bases 1 to 1028)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Data.";
            date: "2018-05-15"; volume: "5:180081. doi"; pages: " 
            10.1038/sdata.2018.81"
COMMENT     Sequence Label: pCMV_mEm_4GS_LF82_666
FEATURES             Location/Qualifiers
     source          1..1028
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    873..1006
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(897..916)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     951..970
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 ctcggcatgg acgagctgta caagcgaatt ggtggagggg gcggttccgg tggcggaggt
       61 tcccgaattc gtcgacggag atctgatatg tcagacaaga cagaaaagaa agctggtgcg
      121 ctgcattctg agctgcgcat cgagctgcat acccaatatg ccattaacac ctggagcggg
      181 cggccagcct cggatagcaa gccttcgctc atcggaatgc cgctgttttt ccggctcatc
      241 tcccggatta accgcgattc actggcagat aatccgtggg cggatgcggc cctggtggaa
      301 gtggaaacat tactggagac ggccagccag cagctccaga aatggttgaa tgatatcgat
      361 gccctgatgg cgatgctgcc agctaacgct tccatttccg gcgtcacctc cacctcgccg
      421 ctgaatatcg gcgttcacag ccataccccg ctgggatacc gctgcgttta tcttcttgtt
      481 gggtttgatc agcttattct gcgtgtcttc caggctcacc attacggtct gatgtcgttg
      541 aaagagcgca aagcctggtt acatcgtggt agccatcaaa ttcgccgcat tttctcggtt
      601 gccctgcgtt accgttctca ccacgttacc cgccaggaca ttgcctcaaa taacgatgct
      661 gcccgacagg ctatcgaaca atttggggaa ctcgacaggg cgatcctgct gggaacccgc
      721 cgttcccgat tttctccgcc agtaagcgcc gaaagtatta aggcgctgaa agccgccttt
      781 aaacgtctgt cacgcaaaga gaaagcggca aagcaggagg tgcgggatga tccgcaatga
      841 atcggtaccc tcgagaagct tgcggccgcg gggatccaga catgataaga tacattgatg
      901 agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg
      961 atgctattgc tttatttgta accattataa gctgcaataa caagttnaca acannntngc
     1021 attcnttt
//
