LOCUS       sequence-18377-e         745 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 745)
  AUTHORS   Colwill K, Wells CD, Elder K, Goudreault M, Hersi K, Kulkarni S, 
            Hardy WR, Pawson T, Morin GB
  TITLE     Modification of the Creator recombination system for proteomics 
            applications--improved expression by addition of splice sites.
  JOURNAL   BMC Biotechnol. 2006 Mar 6;6:13. doi: 10.1186/1472-6750-6-13.
  PUBMED    16519801
REFERENCE   2  (bases 1 to 745)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2006-03-6"; volume: "6:13. doi"; pages: " 
            10.1186/1472-6750-6-13"
COMMENT     Sequence Label: V624 pDNR MCS BE - Donor
FEATURES             Location/Qualifiers
     source          1..745
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(9..42)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     terminator      complement(119..213)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
ORIGIN
        1 gcgctagcat aacttcgtat agcatacatt atacgaagtt atggggatcc gtcgaccccg
       61 ggaagcttga attccaggta agttagccta ggagatcctg gtcatgacta gtgcttggat
      121 tctcaccaat aaaaaacgcc cggcggcaac cgagcgttct gaacaaatcc agatggagtt
      181 ctgaggtcat tactggatct atcaacagga gtccaagcga gctcgatatc aaattacgcc
      241 ccgccctgcc actcatcgca gtactgttgt aattcattaa gcattctgcc gacatggaag
      301 ccatcacaaa cggcatgatg aacctgaatc gccagcggca tcagcacctt gtcgccttgc
      361 gtataatatt tgcccatggt gaaaacgggg gcgaagaagt tgtccatatt ggccacgttt
      421 aaatcaaaac tggtgaaact cacccaggga ttggctgaga cgaaaaacat attctcaata
      481 aaccctttag ggaaataggc caggttttca ccgtaacacg ccacatcttg cgaatatatg
      541 tgtagaaact gccggaaatc gtcgtggtat tcactccaga gcgatgaaaa cgtttcagtt
      601 tgctcatgga aaacggtgta acaagggtga acactatccc atatcaccag ctcaccgtct
      661 ttcattgcca tacgaaattc cggatgagca ttcatcaggc gggcaagaat gtgaataaag
      721 gccggataaa acttgtgctt atttt
//
