LOCUS       sequence-183770-         983 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of E. coli LF82_673 CDS fused to mEmerald.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 983)
  AUTHORS   Collins A, Huett A
  TITLE     A multi-phenotypic imaging screen to identify bacterial effectors by
            exogenous expression in a HeLa cell line.
  JOURNAL   Sci Data. 2018 May 15;5:180081. doi: 10.1038/sdata.2018.81.
  PUBMED    29762554
REFERENCE   2  (bases 1 to 983)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Data.";
            date: "2018-05-15"; volume: "5:180081. doi"; pages: " 
            10.1038/sdata.2018.81"
COMMENT     Sequence Label: pCMV_mEm_4GS_LF82_673
FEATURES             Location/Qualifiers
     source          1..983
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    825..959
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(849..868)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     903..922
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 ctcggcatgg acgagctgta caagcgaatt ggtggagggg gcggttccgg tggcggaggt
       61 tcccgaattc gtcgacggag atctgatatg aaaaagctca aagttgtgtt gttgtcggga
      121 gtgttgacag tgtcaacagt cgcactcgct gcaccttcag tgaatcagga aaacagcctg
      181 acggtgaata cgtcgttaca gatgctggaa cagcaacaaa tgacgcggag cgcacaggag
      241 tgggggctga cagcggaaga gtggcagcgt tatgaaacac tgaaaaaagg acggcgcggc
      301 gtgctgtctc cggggttaga tcccctgacg atgctgggca tcgaggcgcg tagtgacgaa
      361 gaacgtcgcc atttcgctga actggcggtg aagcaggagt ttcagcgggt tgaagcggaa
      421 ctggcatttc agcgggaagt gaacagcgcg tggacgcggg tctatcccgg tgttctgccc
      481 gttcaggatc tgcggagtga agtcagtaac gcccgtcagg cgttgtttgt gaaaggcagc
      541 tgtccggcct gcgagcgcaa gctgactcag ttgatgaaat cgaaccagcc gctggatatc
      601 tacctcgttg atagcggagg taaggatgag acggtccgaa actgggcgaa gaagcacaat
      661 atcccggctg aaaaggtgaa atcccgcctg atcacgctga accacgacaa tggtatgtgg
      721 ctaaaatatg gcagtgggct gatgccggtt gtattacaac aggggccgca gggatggcag
      781 cgcgtaaact gaatcggtac cctcgagaag cttgcggccg cggggatcca gacatgataa
      841 gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt
      901 gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta
      961 acaacaacaa ttgcattcat ttt
//
