LOCUS       sequence-183772-        1013 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of E. coli LF82_678 CDS fused to mEmerald.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1013)
  AUTHORS   Collins A, Huett A
  TITLE     A multi-phenotypic imaging screen to identify bacterial effectors by
            exogenous expression in a HeLa cell line.
  JOURNAL   Sci Data. 2018 May 15;5:180081. doi: 10.1038/sdata.2018.81.
  PUBMED    29762554
REFERENCE   2  (bases 1 to 1013)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Data.";
            date: "2018-05-15"; volume: "5:180081. doi"; pages: " 
            10.1038/sdata.2018.81"
COMMENT     Sequence Label: pCMV_mEm_4GS_LF82_678
FEATURES             Location/Qualifiers
     source          1..1013
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(908..927)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     962..981
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 tcggcatgga cgagctgtac aagcgaattg gtggaggggg cggttccggt ggcggaggtt
       61 cccgaattcg tcgacggaga tctgatatgt cgcagatgaa cacgtcttca cccccggtac
      121 agtcgtcaac gccaccaaag ccgcacggtt tttttaccgt actgctgtgg gatacgccga
      181 tgaacattat cggcatgctg ctggtttcgt tattattcag tgtaattgtg gagttcatcg
      241 gtattgcgtt cttttggcct gatcagggag cggaacatag ccgcgccgtt atgctgacgg
      301 agagcggtta ttttgccgaa ggatttacgc aaagtttgct gctgtcatcg ccggtggtga
      361 ccgtacagag ctggatcgcg ctgggttatc agtggttttt tgtgaatagc ggttttactg
      421 gctggttgca gtcgcttcag acgacgactg ccggaggcgg taatggcgtt acggatgaac
      481 ttaacgcctg gtgtgcctgg gcagtacaga ttgcatatga atacctgatg gcgacgatgt
      541 atgtgtcgct ggtcttcctg atgcgggtca ccattctggt gctgtcggtg ccgctatttg
      601 ttctggttgc cattgtgggg atcgcggatg ggatggttcg acgcgatctc cgtcgctatg
      661 gtgctgcgta tgagtcgagc ttcctctacc atcatgccaa acgcgtggtg aagccagcag
      721 catatattcc gtgcgtattg tatttgtcag cgccgttcgc ggtatatccg aatttgttgc
      781 tgttaccggc agcattgctg atggggctgg cgatttcagt gacgatgggg agttttaaga
      841 agtatttgtg aatcggtacc ctcgagaagc ttgcggccgc ggggatccag acatgataag
      901 atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg
      961 tgaaatttgt gatgctattg ctttatttnt aaccattata agctgcanta aac
//
