LOCUS       sequence-183773-         955 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of E. coli LF82_684 CDS fused to mEmerald.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 955)
  AUTHORS   Collins A, Huett A
  TITLE     A multi-phenotypic imaging screen to identify bacterial effectors by
            exogenous expression in a HeLa cell line.
  JOURNAL   Sci Data. 2018 May 15;5:180081. doi: 10.1038/sdata.2018.81.
  PUBMED    29762554
REFERENCE   2  (bases 1 to 955)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Data.";
            date: "2018-05-15"; volume: "5:180081. doi"; pages: " 
            10.1038/sdata.2018.81"
COMMENT     Sequence Label: pCMV_mEm_4GS_LF82_684
FEATURES             Location/Qualifiers
     source          1..955
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    777..911
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(801..820)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     855..874
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 ctcggcatgg acgagctgta caagcgaatt ggtggagggg gcggttccgg tggcggaggt
       61 tcccgaattc gtcgacggag atctgatatg agtaagttta aaaatgccct gaatgcccgg
      121 gatcaccata tccggacatt acgggcggga tgcggggttt tactggtgct gttggttctg
      181 gcattgtccg gctggatgat gtctccgcgc aatctgacca ttcacaatcc ccccgatctc
      241 cgctccggca gctcccgtcc gtggtgggaa atccctccct caacggtgta tgccttcagc
      301 ttttatatct tccagcaact gaacagctgg ccgaaggatg gtgacgttga ttatccctat
      361 cgtattgaga cgttgtcggc ctacctgaca ccgacctgtc aggaactgct gcataaggat
      421 gcgaagcagc gtaaggacct gggcgaatcg cgggatcgcg tgcgcggtgt atcggaaatc
      481 cctgaacggg gctaccggga tgacagcgtc accattattg accgcgataa ctggcaggtt
      541 cgtctggatt tagtcaccga tgaatatgtt aactccgagc cggttaaacg ctcgctggtg
      601 cgctatccgc tgaaagtggt gcgctgggat aaaaatccgg aggctaaccc gtttggtctg
      661 gcgctggatt gtctctcgga agcgccgcag cgtctggaag cagcccctgt ggttcaggaa
      721 gagaaaaagg gagtgtttaa ctgaatcggt accctcgaga agcttgcggc cgcggggatc
      781 cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa
      841 aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca
      901 ataaacaagt taacaacaac aattgcattc attttatgtt tcnnntcngg ggagg
//
