LOCUS       sequence-183774-        1036 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of E. coli LF82_687 CDS fused to mEmerald.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1036)
  AUTHORS   Collins A, Huett A
  TITLE     A multi-phenotypic imaging screen to identify bacterial effectors by
            exogenous expression in a HeLa cell line.
  JOURNAL   Sci Data. 2018 May 15;5:180081. doi: 10.1038/sdata.2018.81.
  PUBMED    29762554
REFERENCE   2  (bases 1 to 1036)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Data.";
            date: "2018-05-15"; volume: "5:180081. doi"; pages: " 
            10.1038/sdata.2018.81"
COMMENT     Sequence Label: pCMV_mEm_4GS_LF82_687
FEATURES             Location/Qualifiers
     source          1..1036
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    522..656
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(546..565)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     600..619
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(767..783)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(767..783)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(780..802)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    791..807
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(815..821,822..839,840..845))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    860..881
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 ctcggcatgg acgagctgta caagcgaatt ggtggagggg gcggttccgg tggcggaggt
       61 tcccgaattc gtcgacggag atctgatatg aaaggatacg gtttattcat gctggtcgcg
      121 ttgttgctga gcggttgcag tacctccaaa gatgaaatgc tgccacccgg tgacagtacc
      181 atgctcgata tgtggcagaa caaagccagc agcgcccgga ccacgacaga agcgcgtagc
      241 gtgttacgcc gggggctgac ggattcggat ctggcaaccc gtcagtcact ggaagagagc
      301 tatacccgcg atgctcagaa tgagatccag cagcttttcc cgcgcctgcc taacccggat
      361 atggtgattt atatctatcc gcatatggca ggaaatgagc ctgcgccggt gccgggatac
      421 agctcggtct tctctttcta tagccgggta cagtatgcat taccgggcga acggatggag
      481 gcactctgaa tcggtaccct cgagaagctt gcggccgcgg ggatccagac atgataagat
      541 acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg
      601 aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca
      661 acaacaattg cattcatttt atgtttcagg ttcaggggga ggtgtgggag gttttttcgg
      721 atcctctaga gtcgatctgc aggcatgcta gcttggcgta atcatggtca tagctgtttc
      781 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
      841 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
      901 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
      961 ggagaggcgg tttgcgtatt gnngctcttc ncttcnngct cactgactcg ctgcgctcgg
     1021 ncnncggctg cggcga
//
