LOCUS       sequence-18378-e         735 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 735)
  AUTHORS   Colwill K, Wells CD, Elder K, Goudreault M, Hersi K, Kulkarni S, 
            Hardy WR, Pawson T, Morin GB
  TITLE     Modification of the Creator recombination system for proteomics 
            applications--improved expression by addition of splice sites.
  JOURNAL   BMC Biotechnol. 2006 Mar 6;6:13. doi: 10.1186/1472-6750-6-13.
  PUBMED    16519801
REFERENCE   2  (bases 1 to 735)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2006-03-6"; volume: "6:13. doi"; pages: " 
            10.1186/1472-6750-6-13"
COMMENT     Sequence Label: V677 pDNR MCS BE lacZ alpha - Donor
FEATURES             Location/Qualifiers
     source          1..735
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    119..140
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(155..160,161..178,179..185)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     primer_bind     198..220
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     217..233
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     217..233
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             229..402
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEART
                     DRPSQQLRSLNGE"
     primer_bind     complement(249..266)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(249..265)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(258..280)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      complement(464..558)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
ORIGIN
        1 ggatccgtcg acggcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
       61 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
      121 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
      181 tgttgtgtga aattgttagc ggataacaat ttcacacagg aaacagctat gaccatgatt
      241 acggattcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa
      301 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc
      361 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggcgaag cttgaattcc
      421 aggtaagtta gcctaggaga tcctggtcat gactagtgct tggattctca ccaataaaaa
      481 acgcccggcg gcaaccgagc gttctgaaca aatccagatg gagttctgag gtcattactg
      541 gatctatcaa caggagtcca agcgagctcg atatcaaatt acgccccgcc ctgccactca
      601 tcgcagtact gttgtaattc attaagcatt ctgccgacat ggaagccatc acaaacggca
      661 tgatgaacct gaatcgccag cggcatcagc accttgtcgc cttgcgtata atatttgccc
      721 atggtgaaaa cgggg
//
