LOCUS       sequence-183781-        1054 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of E. coli LF82_723 CDS fused to mEmerald.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1054)
  AUTHORS   Collins A, Huett A
  TITLE     A multi-phenotypic imaging screen to identify bacterial effectors by
            exogenous expression in a HeLa cell line.
  JOURNAL   Sci Data. 2018 May 15;5:180081. doi: 10.1038/sdata.2018.81.
  PUBMED    29762554
REFERENCE   2  (bases 1 to 1054)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Data.";
            date: "2018-05-15"; volume: "5:180081. doi"; pages: " 
            10.1038/sdata.2018.81"
COMMENT     Sequence Label: pCMV_mEm_4GS_LF82_723
FEATURES             Location/Qualifiers
     source          1..1054
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    753..887
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(777..796)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     831..850
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 ctcggcatgg acgagctgta caagcgaatt ggtggagggg gcggttccgg tggcggaggt
       61 tcccgaattc gtcgacggag atctgatatg aaaattcaaa acaaacatgt tattgcctgg
      121 ctggagagtt gtgctgcaca tcttactgaa caacaagatt ttctaaccgc acttgacaga
      181 gatattggtg atgccgacca cggcctcaat atgaatcgtg gtttcagtgc cgtgaaagcg
      241 acgttgcccg acattgaacg tcagcatatt ggcaatatcc ttaaaaatac tggcatgaaa
      301 ctactttcca gcgttggcgg tgccagtggt cctctttacg gcacattatt tattcgcgct
      361 tctgcggcag tgggagccag aacagaatta agtctggaag agtggctggc gtgtctggaa
      421 gaagggattg caggcgttat cgcacgagga aaagcagaac aaggggataa aacactgtgt
      481 gatgtctggg ttccagtcct ccacgaagcc aaaaagaacc tgcaagccgg aatgtcgcct
      541 tcccttctgc tgaatacgat ggtgcaagat gcggcaacag ccgtcgataa caccatcaat
      601 atgcaggcga aaaaaggacg tgccagttat ctcggcgctc gcagtgtggg ccatcaggat
      661 ccaggcgcga cctcctcctg gctgatgatt aaggctatgc aggaaggatt agcgggatga
      721 atcggtaccc tcgagaagct tgcggccgcg gggatccaga catgataaga tacattgatg
      781 agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg
      841 atgctattgc tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt
      901 gcattcattt tatgtttcnn ntcnggggag gtgtggnagg ttttttcgga tcctctanag
      961 tcgatctgca ggcatgctag cttggnnnaa tcatggncat agcnnnttnc tgnnntgaaa
     1021 ttntnatccg ctcacnatnc cnnacaacat acga
//
