LOCUS       sequence-183784-         995 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of E. coli LF82_731 CDS fused to mEmerald.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 995)
  AUTHORS   Collins A, Huett A
  TITLE     A multi-phenotypic imaging screen to identify bacterial effectors by
            exogenous expression in a HeLa cell line.
  JOURNAL   Sci Data. 2018 May 15;5:180081. doi: 10.1038/sdata.2018.81.
  PUBMED    29762554
REFERENCE   2  (bases 1 to 995)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Data.";
            date: "2018-05-15"; volume: "5:180081. doi"; pages: " 
            10.1038/sdata.2018.81"
COMMENT     Sequence Label: pCMV_mEm_4GS_LF82_731
FEATURES             Location/Qualifiers
     source          1..995
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(927..946)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 ctcggcatgg acgagctgta caagcgaatt ggtggagggg gcggttccgg tggcggaggt
       61 tcccgaattc gtcgacggag atctgatatg gcaaagtttg cagcaaattt atctatgctc
      121 tttaccgagt atccatttat tgaacgtttt gcacaggcaa gtcacgcagg attccacggc
      181 gttgagtatc ttttccccta tgacttttcc gcagatgagt tggtttcgca actgcatcaa
      241 cataatctga ctcaggtttt gtttaatctt cctgcgggta actggcaaga gggtgaacgg
      301 ggtattgcat gtcatcctgg tcgggcaaaa gaatttcagg agggtgtttg cagagcgata
      361 gaatatgccc aggcccttaa ttgttcacaa gttaattgcc ttgcaggaaa gcatccaggt
      421 ggttattcgc atgaacaatg tcatgaaaca ctggtcgaaa atttgcgcta tgcagtagat
      481 aaattggctt ctcatggcat aaagttagtt ctggaagcta ttaatacaaa agatattccc
      541 ggtttttttg ttaacaacac cagacaagct ctcaatatta ttcatgatgt aaatcatcct
      601 gattttcgct atcaatacga tatttttcat atgcaaatca tggaagggaa tattgctaca
      661 acaatcaaaa acaacctgaa taacattgga catattcaat tagccgataa ccctgggcgt
      721 catgaaccag ggacgggaga aattaattac ccgtggttgt taaattatat agaccagatt
      781 ggttatcagg gatggattgg ttgcgaatat gttccatcta cgacaacgac tgaaagtcta
      841 cgctggctaa aaaacgagac tcaattttga atcggtaccc tcgagaagct tgcggccgcg
      901 gggatccaga catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt
      961 gaaaaaaatg ctttatttgt gaaatttgtg atgct
//
