LOCUS       sequence-183788-        1011 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of E. coli LF82_779 CDS fused to mEmerald.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1011)
  AUTHORS   Collins A, Huett A
  TITLE     A multi-phenotypic imaging screen to identify bacterial effectors by
            exogenous expression in a HeLa cell line.
  JOURNAL   Sci Data. 2018 May 15;5:180081. doi: 10.1038/sdata.2018.81.
  PUBMED    29762554
REFERENCE   2  (bases 1 to 1011)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Data.";
            date: "2018-05-15"; volume: "5:180081. doi"; pages: " 
            10.1038/sdata.2018.81"
COMMENT     Sequence Label: pCMV_mEm_4GS_LF82_779
FEATURES             Location/Qualifiers
     source          1..1011
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    297..431
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(321..340)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     375..394
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(542..558)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(542..558)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(555..577)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    566..582
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(590..596,597..614,615..620))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    635..656
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(773..790)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ctcggcatgg acgagctgta caagcgaatt ggtggagggg gcggttccgg tggcggaggt
       61 tcccgaattc gtcgacggag atctgatatg ttaaaacatc gttatgcaaa tacggaagtg
      121 aaagttactc acagcacatt gaataaacgg tatgatgaag aaattgcaaa caacacaaca
      181 aggagccacg catggaaatg ctcgaagagc accgctgttt tgaaggctgg cagcaacgct
      241 ggcgacacga ctccagtacc ttgaatcggt accctcgaga agcttgcggc cgcggggatc
      301 cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa
      361 aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca
      421 ataaacaagt taacaacaac aattgcattc attttatgtt tcaggttcag ggggaggtgt
      481 gggaggtttt ttcggatcct ctagagtcga tctgcaggca tgctagcttg gcgtaatcat
      541 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
      601 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
      661 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
      721 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
      781 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
      841 taatacggtt atccacagaa tcnnnnataa cgcaggaaag aacatgtgag caaaangnca
      901 gcaaaaggnc aggaaccgta aaaaggccnc gttgctggcg tttttccatn ggctccgccc
      961 ccctgacnag catcacaaaa atcgacnctc aagtcanaag ntggcgaanc c
//
